Development and validation of a method for the measurement of metolachlor mercapturate in urine.
Striley-CAF; Biagini-RE; Mastin-P
Abstr Pap - Am Chem Soc 1997 Sep; 214(Part 1):45-AGRO
Commercially available ELISA (enzyme-linked immunosorbent assay) kits, developed for the detection of metolachlor in ground water, have been applied to the detection of metolachlor in urine. In humans, metolachlor is metabolized to its mercapturic acid derivative. Cross-reactivity tests using synthetic metolachlor mercapturate (MM) have shown that the available kits do not detect this metabolite. In order to develop a method for the detection of MM in urine, polyclonal antibodies were prepared from KLH (keyhole limpet hemocyanin) and BSA (bovine serum albumin) MM conjugates. MM substitution of the respective proteins was analyzed by chemical and instrumental methods. The anti-MM protein antibodies were then used in the development of an ELISA method. The prepared antibodies were also used in an IAC (immunoaffinity chromatography) method. IAC purified urine samples were analyzed by HPLC (high-performance liquid chromatography) with fluorescence detection of the methylmethoxycoumarin ester of MM.
Urinalysis; Metabolites; Proteins; Chromatographic-analysis; Urine-chemistry
Abstracts of Papers - American Chemical Society