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Lipopolysaccharide (LPS)-induced effects on Na+ transport and inflammatory response-gene transcription in the guinea-pig airway epithelium.

Authors
Dodrill-M; Rao-MK; Meighan-T; Fedan-JS
Source
FASEB J 2008 Mar; 22(3)(1):934.1
NIOSHTIC No.
20033739
Abstract
Earlier, we reported that systemic administration of LPS hyperpolarizes transepithelial potential difference (Vt). The increase in Vt was abolished by amiloride and inhibited by indomethacin. These findings suggested an increase in inflammatory-mediator regulated Na+ transport. We investigated the effects of LPS on the mRNA levels of the epithelial sodium channel subunit (ENaC), cyclooxygenase (COX) 2, endothelial nitric oxide synthase (eNOS), interleukin (IL)-1beta, inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)alpha, which regulate ENaC activity and turnover, using real-time RT-PCR. Guinea pigs received 4 mg/kg LPS (i.p.); epithelial cells (EC) and alveolar macrophages (AM) were examined after 3 or 18 h. EC COX2 increased by 36+/-26-fold at 3 h and was elevated by 15+/-12-fold at 18 h. In EC, TNFalpha, IL-1beta and iNOS were increased at 3 h (32+/-17-, 25+/-12- and 7+/-5-fold, respectively) and decreased to baseline after 18 h. AM COX2 was elevated at 3 (163+/-112-fold) and 18 h (8+/-7-fold). AM IL-1beta and AM TNFalpha were elevated at 3 h (127+/-60- and 20+/-7-fold, respectively), and returned to baseline after 18 h. In contrast, AM eNOS increased by 4.7+/-5.4-fold only at 18 h. The other transcripts were not increased more than 4-fold in either tissue at 3 or 18 h. Thus, ENaC mRNA was not affected by LPS. An increase in ENaC transcription cannot explain the LPS-induced increase in Vt.
Keywords
Tumorigenesis; Tumor-inhibition; Laboratory-animals; Animal-studies; Animals
CODEN
FAJOEC
Publication Date
20080301
Document Type
Abstract; Conference/Symposia Proceedings
Fiscal Year
2008
NTIS Accession No.
NTIS Price
Issue of Publication
3
ISSN
0892-6638
NIOSH Division
HELD
Priority Area
Manufacturing
Source Name
The FASEB Journal. Experimental Biology 2008, April 5 - 9, 2008, San Diego, California
State
WV; CA
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