Biomarker and metabolite analysis by HPLC/MS.
Encyclopedia of Chromatography, 3rd Edition. Cazes J, ed., Boca Raton, FL: Taylor and Francis, Online-supplement, 2008 Jan; :1-10
High-performance liquid chromatography-mass spectrometry (HPLC-MS) is a powerful analytical technique widely used in recent years for the analysis of biomarkers and metabolites. Biomarker determination and quantification, whether metabolic or adducted biomolecules, are commonly used to evaluate exposure and support biomonitoring research, especially in the area of occupational exposure and health. Some of the common problems and strategies of HPLC-MS biomarker analysis involve matrix effects, the use of isotope labeled internal standard compounds and sample clean-up; usually all of these factors must be evaluated within the development phase of an analysis procedure. Specific examples of biomarker analysis using HPLC-MS include acrylamide, aromatic compounds, and 1-bromopropane, and these examples are discussed in detail.
Analytical-processes; Analytical-chemistry; Analytical-methods; Biomarkers; Sampling-methods; Sample-preparation
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Encyclopedia of Chromatography, 3rd Edition