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Assessment of autofluorescent alveolar macrophages and centriacinar emphysema as markers of smoking status in decreased coal miners.

Authors
Vi-LH; Rao-GVS; Fekedulegn-D; Hubbs-AF; Battelli-LA; Kashon-ML; Vallyathan-V; Keshava-N; Ducatman-BS
Source
Mod Path 2003 Jan; 16(1):8A
NIOSHTIC No.
20033686
Abstract
Background: smoking status is often not known in autopsy studies Potential surrogates for this information include severity of centriacinar emphysema (CE) and autofluorescent macrophages. Cigarette smoke contains many gaseous and particulate components, and a strong intracellular fluorescence of these components is present in unfixed alveolar macrophages from smokers and ex-smokers, but has not been studied in paraffin-embedded tissue. Design: using lung tissue from 100 random cases in the National Coal Workers' Autopsy Study, we reviewed the intensity (qualitative, 0-2+) and alveolar area fraction of autofluorescent macrophages (AAFAM) in deparaffinized, unstained sections at three different wavelengths (blue, ultraviolet (DAPI)) at 360-370 nm excitation, green (FITC) at 460-500 nm excitation, and red (Texas RED) at 532.5-5875 nm excitation. Quantitative studies utilized a commercial image analysis system (Melamorph(tradename) Imaging System, Universal Imaging Corporation). In addition, the severity of CE (0-2+) was evaluated by 2 blinded observers, but focal emphysema (associated with coal workers' pneumoconiosis) was not scored as part of this. These results were compared to the next-of-kin reported smoking status using SAS statistical software. Results: CE and smoking status were not significantly associated (p=-O2658) in these miners. Qualitative assessment of autofluorescence intensity correlated with smoking status, as did AAFAM at 532.5-5g7.5 nm excitation (p
Keywords
Alveolar-cells; Lung; Lung-cells; Lung-disorders; Lung-irritants; Particulate-dust; Particulates; Smoking; Coal-dust; Coal-miners; Coal-workers; Pulmonary-disorders; Pulmonary-system
CODEN
MODPEO
Publication Date
20030101
Document Type
Abstract; Conference/Symposia Proceedings
Fiscal Year
2003
NTIS Accession No.
NTIS Price
Issue of Publication
1
ISSN
0893-3952
NIOSH Division
HELD
Source Name
Modern pathology
State
WV
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