Use of labeled single walled carbon nanotubes to study acute translocation from the lungs.
Mercer-RR; Scabilloni-JF; Wang-L; Battelli-LA; Castranova-V
Toxicologist 2008 Mar; 102(1):286
Single walled carbon nanotubes (SWCNT) have a small size and unique physicalchemical properties which suggest that novel particles may translocate to systemic organs after deposition in the lungs. In this study, gold particles (10nm) were used to label a highly dispersed preparation of SWCNT with a mean geometric diameter of 1.1 microns. The gold labeled SWCNT were delivered to the lungs by pharyngeal aspiration to C57BL/6 mice. Neutron activation analysis (NAA) of lung, blood and other organs was carried out at various times after aspiration to determine if the gold labeled SWCNT translocated out of the lungs. Six mice per group were studied at 1 hour, 1 day and 3 days after exposure to a single dose of 50 ug of gold labeled SWCNT. A separate group was given gold particles without SWCNT. A phosphate buffered saline (PBS) aspiration group served as the negative control. At sacrifice the lungs, heart, brain, liver, kidneys and a blood sample were taken for analysis of gold content by NAA. Gold content of the PBS aspiration group was negligible. In the gold labeled SWCNT group lung gold content at 1 day was 65 percent of that at 1 hour and not significantly different from the content at 3 days. The heart, brain, liver, kidney and blood samples from the gold labeled SWCNT groups contained less than 1 part in 200 of the total dose and were not statistically different from the PBS aspiration group at 1 hour, 1 day and 3 days. The results indicate that SWCNT do not readily translocate from the lungs.
Particle-aerodynamics; Particulate-dust; Particulate-sampling-methods; Respirable-dust; Respiratory-hypersensitivity; Respiratory-irritants; Lung-burden; Lung-irritants; Lymphatic-system-disorders; Lung-function; Pulmonary-disorders; Pulmonary-system-disorders; Laboratory-animals; Nanotechnology
The Toxicologist. Society of Toxicology 47th Annual Meeting and ToxExpo, March 16-20, 2008, Seattle, Washington