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Zinc diethyldithiocarbamate allergenicity: potential haptenation mechanisms.

Authors
Chipinda-I; Hettick-JM; Simoyi-RH; Siegel-PD
Source
Toxicologist 2008 Mar; 102(1):270-271
NIOSHTIC No.
20033607
Abstract
Zinc diethyldithiocarbamate (ZDEC) and its disulfide, tetraethylthiuram disulfide (TETD) are rubber accelerant contact allergens that cross-react in some individuals. Rubber gloves are often worn while using oxidizing disinfectants. The present study explored potential protein haptenation mechanisms of ZDEC and its oxidation products. Oxidation of ZDEC by bleach, iodine or hydrogen peroxide resulted in production of TETD, tetraethylthiocarbamoyl disulfide (TETCD) and tetraethyldicarbamoyl disulfide (TEDCD). Cysteine, glutathione and albumin thiols reduced TETD with subsequent mixed disulfide formation suggesting a potential route of protein haptenation. Chelation of the copper ion bound on the active site of the monomeric Cu/Zn superoxide dismutase (SOD) by diethyldithiocarbamate was observed when ZDEC was reacted with SOD. The specificity of the ZDEC-Cu(II) reaction and lack of ZDEC reactivity with reduced Cys6 and Cys111 on SOD suggested formation of the DEC-Cu(II) co-ordinate bond as another haptenation mechanism. The murine local lymph node assay (LLNA) was used to evaluate sensitization potential of ZDEC, Cobalt-DEC (CoDEC), TETD and TEDCD. The ranking of the sensitization potency was ZDEC >or= TEDCD > TETD >> CoDEC (no sensitization achieved) with EC3 values 1.01, 1.70, 5.42 and >7.5 respectively. CoDEC does not bind to Cu-proteins or form mixed disulfides with free thiols demonstrating the importance of these properties in ZDEC sensitization. Paradoxically, oxidative substitution of S by O on the thiocarbamates of TETD (= TEDCD) was allergenic, but was not subject to haptenation through mixed disulfides or metal chelation. It is concluded that ZDEC may haptenize proteins through (1) chelation of metals on metalloproteins, (2) TETD mediated formation of mixed disulfides, and (3) possibly through nucleophilic addition onto an TEDCD electrophilic carbamoyl group.
Keywords
Metallic-minerals; Skin-absorption; Skin-exposure; Skin-irritants; Skin-sensitivity; Chemical-hypersensitivity; Chemical-properties; Chelating-agents; Oxidation; Oxidative-metabolism; Oxidative-processes; Oxidative-enzymes
Publication Date
20080301
Document Type
Abstract
Fiscal Year
2008
NTIS Accession No.
NTIS Price
Issue of Publication
1
ISSN
1096-6080
NIOSH Division
HELD
Priority Area
Healthcare and Social Assistance; Services
Source Name
The Toxicologist. Society of Toxicology 47th Annual Meeting and ToxExpo, March 16-20, 2008, Seattle, Washington
State
WV; OR; WA
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