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Analysis of immunotoxicity by enumeration of antibody-producing B cells.

Authors
Anderson-SE; Munson-AE; Mead-BJ
Source
Current Protocols in Toxicology. Costa LG, Hodgson E, Reed DJ, Maines MD, Sassa S, Sipes IG, Bradfield C, Lawrence DA, Morgan KS, eds., New York: Wiley and Sons, Inc., 2004 Jan; 2(Suppl 20):18.11.1-18.11.19
NIOSHTIC No.
20033365
Abstract
The plaque-forming cell (PFC) assay measures the humoral immune response mediated by the concerted actions of antigen-presenting cells, T lymphocytes, and B lymphocytes. The most common form of the plaque method is used for the detection of murine primary IgM antibodies directed against the T cell-dependent sheep red blood cell (sRBC) surface antigens. Research has shown that the PFC response to sRBC is not only an excellent monitor of the primary effector function of the B cell, but that it may be the most sensitive immune parameter currently available to identify chemical perturbation. Several modifications and variations of the PFC assay are described in detail in this unit.
Keywords
Diseases; Risk-analysis; Risk-factors; Immune-system; Infectious-diseases; Immunotoxins; Immunological-tests; Epidemiology; Quantitative-analysis; Models; Immunoglobulins; Qualitative-analysis; Immune-system-disorders; Immune-system; Cell-culture-techniques; Cell-function; Chemical-properties
Publication Date
20060801
Document Type
Book or book chapter
Editors
Costa-LG; Hodgson-E; Reed-DJ; Maines-MD; Sassa-S; Sipes-IG; Bradfield-C; Lawrence-DA; Morgan-KS
Fiscal Year
2006
NTIS Accession No.
NTIS Price
ISBN No.
0555019802
NIOSH Division
HELD
Source Name
Current Protocols in Toxicology
State
WV
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