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Alteration of deposition pattern and pulmonary response as a result of improved dispersion of aspirated single-walled carbon nanotubes in a mouse model.

Authors
Mercer-RR; Scabilloni-J; Wang-L; Kisin-E; Murray-AR; Schwegler-Berry-D; Shvedova-AA; Castranova-V
Source
Am J Physiol, Lung Cell Mol Physiol 2008 Jan; 294(1):L87-L97
NIOSHTIC No.
20033223
Abstract
Nanoparticles have a fundamental dimension of <100 nm. However, on suspension in media, agglomerates of nanoparticles are the more common structure. This is particularly evident in prior intratracheal instillation or aspiration studies of single-walled carbon nanotubes (SWCNT), in which granulomatous lesions encased by epithelioid macrophages were produced by large agglomerates. In this study, we tested the hypothesis of whether exposure to more dispersed SWCNT structures would alter pulmonary distribution and response. A dispersed preparation of single-walled carbon nanotubes (DSWCNT) with a mean diameter of 0.69 mu m was given by pharyngeal aspiration to C57BL/6 mice. Electron microscopy demonstrated a highly dispersed, interstitial distribution of DSWCNT deposits by 1 day postexposure. Deposits were generally <1 mu m. Macrophage phagocytosis of DSWCNT was rarely observed at any time point. Lung responses were studied by lavage and morphometry at 1 h, 1 day, 7 day, and 1 mo after a single DSWCNT exposure of 10 mu g/mouse. Lung sections and lavage cells demonstrated an early, transient neutrophilic and inflammatory phase that rapidly resolved and was similar to that observed with large agglomerates. No granulomatous lesions or epithelioid macrophages were detected. Morphometric measurement of Sirius red staining was used to assess the connective tissue response. The average thickness of connective tissue in alveolar regions was 0.10 +/- 0.02, 0.09 +/- 0.02, 0.10 +/- 0.01, 0.48 +/- 0.04, and 0.88 +/- 0.19 mu m for PBS and 1-h, 1-day, 7-day, and 1-mo postexposure groups, respectively. The results demonstrate that dispersed SWCNT are rapidly incorporated into the alveolar interstitium and that they produce an increase in collagen deposition.
Keywords
Lung; Lung-cells; Cell-biology; Cell-function; Cell-morphology; Cellular-reactions; Dust-analysis; Dust-counters; Dust-exposure; Particle-aerodynamics; Particulate-dust; Particulates; Biological-monitoring; Biological-transport; Statistical-analysis; Nanotechnology
Contact
R. R. Mercer, Pathology and Physiology Research Branch, Health Effect Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505
CODEN
APLPE7
Publication Date
20080101
Document Type
Journal Article
Email Address
rmercer@cdc.gov
Fiscal Year
2008
NTIS Accession No.
NTIS Price
Issue of Publication
1
ISSN
1040-0605
NIOSH Division
HELD
Priority Area
Manufacturing
Source Name
American Journal of Physiology: Lung Cellular and Molecular Physiology
State
WV
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