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Increasing or stabilizing renal epoxyeicosatrienoic acid production attenuates abnormal renal function and hypertension in obese rats.

Authors
Huang-H; Morisseau-C; Wang-J; Yang-T; Falck-JR; Hammock-BD; Wang-MH
Source
Am J Physiol Renal Physiol 2007 Jul; 293(1):F342-F349
NIOSHTIC No.
20033134
Abstract
Since epoxyeicosatrienoic acids (EETs) affect sodium reabsorption in renal tubules and dilate the renal vasculature, we have examined their effects on renal hemodynamics and sodium balance in male rats fed a high-fat (HF) diet by fenofibrate, a peroxisome proliferator-activated receptor-alpha (PPAR-alpha) agonist and an inducer of cytochrome P-450 (CYP) epoxygenases; by N-methanesulfonyl-6-(2-proparyloxyphenyl)hexanamide (MSPPOH), a selective EET biosynthesis inhibitor; and by 12-(3-adamantane-1-yl-ureido)dodecanoic acid (AUDA), a selective inhibitor of soluble epoxide hydrolase. In rats treated with fenofibrate (30 mg.kg(-1).day(-1) ig) or AUDA (50 mg/l in drinking water) for 2 wk, mean arterial pressure, renal vascular resistance, and glomerular filtration rate were lower but renal blood flow was higher than in vehicle-treated control rats. In addition, fenofibrate and AUDA decreased cumulative sodium balance in the HF rats. Treatment with MSPPOH (20 mg.kg(-1).day(-1) iv) + fenofibrate for 2 wk reversed renal hemodynamics and sodium balance to the levels in control HF rats. Moreover, fenofibrate caused a threefold increase in renal cortical CYP epoxygenase activity, whereas the fenofibrate-induced elevation of this activity was attenuated by MSPPOH. Western blot analysis showed that fenofibrate induced the expression of CYP epoxygenases in renal cortex and microvessels and that the induction effect of fenofibrate was blocked by MSPPOH. These results demonstrate that the fenofibrate-induced increase of CYP epoxygenase expression and the AUDA-induced stabilization of EET production in the kidneys cause renal vascular dilation and reduce sodium retention, contributing to the improvement of abnormal renal hemodynamics and hypertension in HF rats.
Keywords
Animal-studies; Animals; Kidney-cells; Kidney-disorders; Kidney-function; Kidney-toxins; Kidneys; Hypertension; Chemical-hypersensitivity; Chemical-inhibition; Chemical-synthesis; Weight-factors; Weight-measurement; Electrolytes; Electrolytic-analysis; Blood-analysis; Blood-pressure; Blood-samples; Blood-sampling; Sodium-compounds
Contact
H. Huang, Dept. of Physiology, Medical College of Georgia, Augusta, GA 30912
Publication Date
20070701
Document Type
Journal Article
Email Address
mwang@mail.mcg.edu
Funding Type
Agriculture; Cooperative Agreement
Fiscal Year
2007
NTIS Accession No.
NTIS Price
Identifying No.
Cooperative-Agreement-Number-U50-OH-007550
Issue of Publication
1
ISSN
1931-857X
Source Name
American Journal of Physiology: Renal Physiology
State
CA; GA; TX; UT
Performing Organization
University of California - Davis
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