Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Inflammatory properties of iron-containing carbon nanoparticles.

Authors
Waldman-WJ; Kristovich-R; Knight-DA; Dutta-PK
Source
Chem Res Toxicol 2007 Aug; 20(8):1149-1154
NIOSHTIC No.
20032938
Abstract
Inflammatory responses following exposure of carbon nanoparticles to human macrophage and endothelial cells were employed as indicators of particulate biological activity. Hundred nanometer carbon particles (nC) with and without nonextractable surface-bound iron were synthesized using a templating approach, and human monocyte-derived macrophages (MDM) were exposed to various concentrations of these particulates. Supernatants recovered from MDM 24 h postexposure were assayed for the inflammatory cytokine tumor necrosis factor-alpha (TNFalpha) by a quantitative ELISA and tested for their ability to induce expression of intercellular adhesion molecule-1 (ICAM-1) on human endothelial cells (EC) by immunofluorescence flow cytometry. Data generated by these experiments demonstrated that nC-Fe was far more biologically active than nC. In addition, the chemical reactivity of nC-Fe toward decomposition of hydrogen peroxide to form hydroxyl radicals was significantly higher than that of nC and correlated well with the increase in the strength of the inflammatory response, though a direct proof of creation of hydroxyl radicals in the biological system is not provided. Comparison with micrometer-sized carbon and carbon-iron particles suggests that the chemical and biological reactivity is correlated with surface area.
Keywords
Particulates; Particulate-dust; Aerosols; Aerosol-particles; Iron-compounds; Cell-cultures; Cell-function; Cellular-function; Nanotechnology
Contact
James Waldman, Department of Pathology, The Ohio State University College of Medicine, 4160 Graves Hall, 333 West 10th Avenue, Columbus, OH 43210
CODEN
CRTOEC
CAS No.
7439-89-6
Publication Date
20070801
Document Type
Journal Article
Email Address
james.waldman@osumc.edu; dutta.1@osu.edu
Funding Type
Grant
Fiscal Year
2007
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-009141
Issue of Publication
8
ISSN
0893-228X
Source Name
Chemical Research in Toxicology
State
OH
Performing Organization
The Ohio State University
TOP