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Negative ion chemical ionization mass spectrometry for the analysis of 3,5,6-trichloro-2-pyridinol in saliva of rats exposed to chlorpyrifos.

Authors
Campbel-JA; Timchalk-C; Kousba-AA; Wu-H; Valenzuela-BR; Hoppe-EW
Source
Anal Lett 2005 Jun; 38(6):939-949
NIOSHTIC No.
20032918
Abstract
Organophosphorus (OP) insecticides (e.g. chlorpyrifos) are widely used in a variety of applications, and the potential exists for significant occupational and environmental exposures. They have been associated with more occupational poisoning cases than any other class of insecticides. One of the best approaches for accurately assessing human dosimetry and determining risk from both occupational and environmental exposure is biomonitoring. Biological matrices such as blood and urine have been routinely used for biomonitoring; however, other matrices such as saliva represent a simple and readily obtainable fluid. As a result, saliva has been suggested as an alternative biological matrix for the evaluation of a broad range of biomarkers such as environmental contaminants, drugs of abuse, hormones, chemotherapeutics, heavy metals, and pesticides. Chlorpyrifos (CPF), and its major metabolite, 3,5,6-trichloro-2-pyridinol (TCP), have been quantified in urine and blood as a biomarker for exposure to OP insecticides. The purpose of this study was to develop an analytical approach for detecting and quantitating the levels of TCP in saliva obtained from rats exposed to CPF and to evaluate the potential of saliva as a noninvasive biomonitoring matrix for the determination of exposure to OP insecticides. Adult male rats were administered CPF, and blood and saliva were humanely collected for analysis of TCP and CPF. The TCP was detected and quantitated in saliva using negative ion chemical ionization mass spectrometry with selected ion monitoring. Initial results indicate that saliva potentially may be utilized as a noninvasive biomonitoring matrix to determine exposure to organophosphate insecticides.
Keywords
Pharmacodynamics; Organo-phosphorus-pesticides; Exposure-levels; Blood-samples; Models; Insecticides; Organo-phosphorus-compounds; Neurotoxic-effects; Neurotoxicity; Neurotoxins; Acute-exposure; Brain-disorders; Brain-function; Pesticides; Work-environment; Biochemistry; Biological-function; Biological-monitoring; Biomarkers; Environmental-contamination; Environmental-exposure; Environmental-factors; Environmental-hazards; Chemotherapy; Hormone-activity; Metal-poisoning; Drug-interaction; Substance-abuse; Urinalysis; Urine-chemistry; Blood-plasma; Blood-analysis; Physiological-chemistry; Physiological-disorders; Physiological-response; Hematology; Insecticide-poisoning
Contact
Battelle Pacific Northwest Division, Chemical Dosimetry, 902 Battelle Blvd., P.O. Box 999, Richland, WA 99352
CODEN
ANALBP
Publication Date
20050601
Document Type
Journal Article
Email Address
charles.timchalk@pnl.gov
Funding Amount
246279
Funding Type
Grant
Fiscal Year
2005
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-003629
Issue of Publication
6
ISSN
0003-2719
Priority Area
Research Tools and Approaches: Exposure Assessment Methods
Source Name
Analytical Letters
State
WA
Performing Organization
Battelle Memorial Institute, Richland, Washington
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