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Bacterial endospore inactivation caused by outgassing of vapourous hydrogen peroxide from polymethyl methacrylate (Plexiglas(R)).

Authors
Baron-PA; Estill-CF; Beard-JK; Hein-MJ; Larsen-L
Source
Lett Appl Microbiol 2007 Nov; 45(5):485-490
NIOSHTIC No.
20032842
Abstract
Aims: To investigate the cause and to eliminate the inactivation of Bacillus anthracis strain Sterne spores settled onto agar and stainless steel surfaces in plastic holders. Methods and Results: In an experimental chamber in which spores settled onto sampling surfaces, vapourous hydrogen peroxide (VHP) was used for decontamination between experiments. It was demonstrated that hydrogen peroxide (H2O2) absorbed into plastic (Plexiglas(R)) surfaces and could outgas in the sample holders. Further experiments demonstrated that H2O2 was released from Plexiglas(R) sample holders in sufficient quantity to inactivate spores. High temperature degassing (30-35 degrees C) for several days or aluminum coating of the surfaces were two remedies found to be effective in preventing inadvertent spore inactivation. Conclusions: H2O2 can be absorbed into plastic and released after an extended period of time (weeks), allowing a sufficient concentration to accumulate in small volumes to inactivate spores. Outgassing the plastic or coating the surface with an impermeable layer are potential solutions to reduce spore inactivation. Significance and Impact of the Study: Many studies with bacilli and other organisms are carried out using small plastic containers that may have been sterilized using H2O2 or other agents. This study presents a cautionary note to ensure elimination of H2O2 or other sterilizing agents to prevent spurious results.
Keywords
Analytical-chemistry; Analytical-processes; Peroxides; Bacteria; Bacterial-cultures; Plastic-products; Plastics; Coatings; Microorganisms
Contact
Paul Baron, NIOSH MSR3, 4676 Columbia Parkway, Cincinnati, OH 45226, USA
CODEN
LAMIE7
CAS No.
7722-84-1; 9011-14-7
Publication Date
20071101
Document Type
Journal Article
Email Address
pbaron@cdc.gov
Fiscal Year
2008
NTIS Accession No.
NTIS Price
Issue of Publication
5
ISSN
0266-8254
NIOSH Division
DART; DSHEFS
Priority Area
Research Tools and Approaches: Surveillance Research Methods; Research Tools and Approaches: Control Technology and Personal Protective Equipment
Source Name
Letters in Applied Microbiology
State
OH
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