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Compensatory mechanism for homeostatic blood pressure regulation in Ephx2 gene-disrupted mice.

Authors
Luria-A; Weldon-SM; Kabcenell-AK; Ingraham-RH; Matera-D; Jiang-H; Gill-R; Morisseau-C; Newman-JW; Hammock-BD
Source
J Biol Chem 2007 Feb; 282(5):2891-2898
NIOSHTIC No.
20032813
Abstract
Arachidonic acid-derived epoxides, epoxyeicosatrienoic acids, are important regulators of vascular homeostasis and inflammation, and therefore manipulation of their levels is a potentially useful pharmacological strategy. Soluble epoxide hydrolase converts epoxyeicosatrienoic acids to their corresponding diols, dihydroxyeicosatrienoic acids, modifying or eliminating the function of these oxylipins. To better understand the phenotypic impact of Ephx2 disruption, two independently derived colonies of soluble epoxide hydrolase-null mice were compared. We examined this genotype evaluating protein expression, biofluid oxylipin profile, tissue oxylipin production capacity, and blood pressure. Ephx2 gene disruption eliminated soluble epoxide hydrolase protein expression and activity in liver, kidney, and heart from each colony. Plasma levels of epoxy fatty acids were increased, and fatty acid diols levels were decreased, while measured levels of lipoxygenase- and cyclooxygenase-dependent oxylipins were unchanged. Liver and kidney homogenates also show elevated epoxide fatty acids. However, in whole kidney homogenate a 4-fold increase in the formation of 20-hydroxyeicosatetraenoic acid was measured along with a 3-fold increase in lipoxygenase-derived hydroxylation and prostanoid production. Unlike previous reports, however, neither Ephx2-null colony showed alterations in basal blood pressure. Finally, the soluble epoxide hydrolase-null mice show a survival advantage following acute systemic inflammation. The data suggest that blood pressure homeostasis may be achieved by increasing production of the vasoconstrictor, 20-hydroxyeicosatetraenoic acid in the kidney of the Ephx2-null mice. This shift in renal metabolism is likely a metabolic compensation for the loss of the soluble epoxide hydrolase gene.
Keywords
Acids; Chemical-analysis; Chemical-composition; Chemical-extraction; Gene-mutation; Genes; Genotoxic-effects; Genotoxicity; Liver; Kidneys; Heart; Plasma-membrane; Fatty-acids; Lipid-disorders; Metabolic-study; Metabolism
Contact
A Luria, Department of Entomology, University of California, Davis, California 95616
CODEN
JBCHA3
Publication Date
20070202
Document Type
Journal Article
Email Address
bdhammock@ucdavis.edu
Funding Amount
1055222
Funding Type
Cooperative Agreement
Fiscal Year
2007
NTIS Accession No.
NTIS Price
Identifying No.
Cooperative-Agreement-Number-U07-CCU-906162
Issue of Publication
5
ISSN
0021-9258
Source Name
Journal of Biological Chemistry
State
CA
Performing Organization
University of California - Davis
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