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Aerosolized fluorescent microspheres detected in the lung using confocal scanning laser microscopy.

Authors
Pinkerton-KE; Gallen-JT; Mercer-RR; Wong-VC; Plopper-CG; Tarkington-BK
Source
Microsc Res Tech 1993 Dec; 26(5):437-443
NIOSHTIC No.
20032749
Abstract
Aerosolized fluorescent microspheres were used to study particle deposition in site-specific regions of the lung with confocal laser scanning microscopy. A nebulizer was used to aerosolize microspheres followed by passage through a heated discharging column to reduce static charge and to remove water surrounding each microsphere. Precoating of microspheres with albumin helped to minimize displacement during vascular fixation of the lungs. Confocal laser microscopy facilitated visualization of microspheres throughout the bronchial tree, ducts, and alveoli of the lungs. The use of fluorescent microspheres and confocal laser imaging provided distinct advantages compared with other methods to study lung particle deposition due to (1) the generation of single microspheres of uniform size by nebulization, (2) easy detection of microspheres in large slabs of microdissected lung tissues, (3) excellent resolution of tissue surfaces and microspheres for an infinite number of orientations and planes of section, and (4) the ability to visualize microspheres below fluid lining layers and on surfaces that could not easily be done by other methods of microscopy.
Keywords
Lung; Lung-disorders; Lung-fibrosis; Lung-irritants; Aerosol-particles; Aerosol-sampling; Scanning-techniques; Lasers; Microscopic-analysis; Microscopy; Particle-aerodynamics; Particle-counters; Particulate-sampling-methods; Tissue-culture; Tissue-disorders; Tissue-distribution
Contact
Kent E. Pinkerton: Department of Anatomy and Cell Biology, California Regional Primate Research Center, School of Veterinary Medicine, University of California, Davis, California 95616
CODEN
MRTEEO
Publication Date
19931201
Document Type
Journal Article
Funding Amount
1055222
Funding Type
Cooperative Agreement
Fiscal Year
1994
NTIS Accession No.
NTIS Price
Identifying No.
Cooperative-Agreement-Number-U07-CCU-906162
Issue of Publication
5
ISSN
1059-910X
Source Name
Microscopy Research and Technique
State
CA; NC
Performing Organization
University of California - Davis
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