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Improved method to disperse nanoparticles for in vitro and in vivo investigation of toxicity.

Authors
Sager-TM; Porter-DW; Robinson-VA; Lindsley-WG; Schwegler-Berry-DE; Castranova-V
Source
Nanotoxicology 2007 Jun; 1(2):118-129
NIOSHTIC No.
20032633
Abstract
Nanoparticles agglomerate and clump in solution, making it difficult to accurately deliver them for in vivo or in vitro experiments. Thus, experiments were conducted to determine the best method to suspend nanosized particles. Ultrafine and fine carbon black and titanium dioxide were suspended in phosphate buffered saline (PBS), rat and mouse bronchoalveolar lavage fluid (BALF), and PBS containing dipalmitoyl phosphatidylcholine (DPPC) and/or mouse serum albumin. To assess and compare how these various suspension media dispersed the nanoparticles, images were taken using light microscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The results of this study show that PBS is not a satisfactory medium to prepare nanoparticle suspensions. However, BALF was an excellent media in which to suspend nanoparticles. The use of PBS containing protein or DPPC alone, in concentrations found in BALF, did not result in satisfactory particle dispersion. However, PBS-containing protein plus DPPC was satisfactory, although less effective than BALF.
Keywords
Risk-analysis; Pulmonary-system-disorders; Respiratory-system-disorders; Analytical-methods; Analytical-processes; Particle-aerodynamics; Particulate-sampling-methods; Particulates; Animal-studies; Nanotechnology
Contact
Vincent Castranova, M/S 2015, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505
Publication Date
20070601
Document Type
Journal Article
Email Address
vic1@cdc.gov
Fiscal Year
2007
NTIS Accession No.
NTIS Price
Issue of Publication
2
ISSN
1743-5390
NIOSH Division
HELD
Priority Area
Manufacturing
Source Name
Nanotoxicology
State
WV
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