A new field-compatible methodology for the collection and analysis of fungal fragments.
Seo-S-C; Grinshpun-SA; Iossifova-Y; Schmechel-D; Rao-CY; Reponen-T
Aerosol Sci Tech 2007 Aug; 41(8):794-803
A field-compatible collection system was developed and tested for the collection and analysis of fungal fragments. The new collection system consists of two types of Sharp-Cut cyclone samplers (PM2.5 and PM1.0) and an after-filter. Fungal particles are collected into three size fractions: (1) spores (>2.5 m); (2) a fragment-spore mixture (1.0-2.5mu m); and (3) submicrometer-sized fragments (<1.0mu m). The system was laboratory-tested using polystyrene latex (PSL) particles and particulate matter aerosolized from sporulating Aspergillus versicolor and Stachybotrys chartarum cultures. In addition to the particle count measured with direct-reading instruments, the (1--> 3)-beta-D-glucan content in each size fraction was determined with the Limulus Amebocyte Lysate (LAL) assay. Experiments conducted with PSL particles showed that the 50% cut-off values of the two cyclone samplers under the test conditions were 2.25 mu m and 1.05 mu m, respectively. No particle bounce onto the after-filter was observed when the total particle number entering the collection system was kept below 1.6 × 108. The (1--> 3)-beta-D-glucan assay of samples aerosolized from both fungal species suggested that surface area is an important factor for determining the (1--> 3)-beta-D-glucan content in the entire size-range of particles. In conclusion, the new methodology is a promising tool for separating and analyzing fungal fragment samples.
Aerosol-particles; Fungi; Particle-aerodynamics; Particulate-sampling-methods; Particulates; Sampling-methods; Molds; Fungal-infections; Fungal-diseases
Tiina Reponen, Center for Health-Related Aerosol Studies, Department of Environmental Health, University of Cincinnati, Cincinnati, OH 45267-0056
Aerosol Science and Technology