Cholinesterase measurements with an automated kit.
Oliveira-GH; Henderson-JD; Wilson-BW
Am J Ind Med 2002 Aug; 42(Suppl 2):49-53
Background : The Test-mate kit determines acetylcholinesterase (AChE, EC 188.8.131.52) and hemoglobin content of a drop of blood, displaying enzyme activities normalized to 25°C. Previous models produced inconsistent results at different temperatures. This report focuses on the current model, ChE 400, and two instruments of a previous OP model. Methods: AChE activities were determined by the Ellman assay, using the three kits and a 96-well microplate reader. Temperatures ranged from 10 to 37°C. Fetal bovine serum was the source of AChE. Results: Normalized activities decreased below 20°C in the ChE model and below 25°C in the OP models. Activities of the same serum sample differed between the three Test-mate kits, ranging from 1.03 to 1.49 moles/min/ml. Percent errors were greater than with the microplate reader at all temperatures. Conclusions: Neither we nor the manufacturer recommend the current Test-mate model for fieldwork. Nevertheless, there have been field measurements with Test-Mate kits, and we recommend that an enzyme activity standard be run in parallel with their use.
Acetylcholinesterase; Blood-analysis; Blood-samples; Blood-sampling; Temperature-control; Temperature-effects; Temperature-measurement; Industrial-hazards; Industrial-safety-programs
Barry W. Wilson, Animal Science Department, Meyer Hall, University of California, 1 Shields Ave., Davis, CA 95616
American Journal of Industrial Medicine
University of California - Davis