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Cholinesterase measurements with an automated kit.

Authors
Oliveira-GH; Henderson-JD; Wilson-BW
Source
Am J Ind Med 2002 Aug; 42(Suppl 2):49-53
NIOSHTIC No.
20032411
Abstract
Background : The Test-mate kit determines acetylcholinesterase (AChE, EC 3.1.1.7) and hemoglobin content of a drop of blood, displaying enzyme activities normalized to 25C. Previous models produced inconsistent results at different temperatures. This report focuses on the current model, ChE 400, and two instruments of a previous OP model. Methods: AChE activities were determined by the Ellman assay, using the three kits and a 96-well microplate reader. Temperatures ranged from 10 to 37C. Fetal bovine serum was the source of AChE. Results: Normalized activities decreased below 20C in the ChE model and below 25C in the OP models. Activities of the same serum sample differed between the three Test-mate kits, ranging from 1.03 to 1.49 moles/min/ml. Percent errors were greater than with the microplate reader at all temperatures. Conclusions: Neither we nor the manufacturer recommend the current Test-mate model for fieldwork. Nevertheless, there have been field measurements with Test-Mate kits, and we recommend that an enzyme activity standard be run in parallel with their use.
Keywords
Acetylcholinesterase; Blood-analysis; Blood-samples; Blood-sampling; Temperature-control; Temperature-effects; Temperature-measurement; Industrial-hazards; Industrial-safety-programs
Contact
Barry W. Wilson, Animal Science Department, Meyer Hall, University of California, 1 Shields Ave., Davis, CA 95616
CODEN
AJIMD8
Publication Date
20020801
Document Type
Journal Article
Email Address
bwwilson@ucdavis.edu
Funding Amount
1055222
Funding Type
Cooperative Agreement
Fiscal Year
2002
NTIS Accession No.
NTIS Price
Identifying No.
Cooperative-Agreement-Number-U07-CCU-906162
ISSN
0271-3586
Source Name
American Journal of Industrial Medicine
State
CA
Performing Organization
University of California - Davis
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