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Oxime reactivation of RBC acetylcholinesterases for biomonitoring.

Authors
Hansen-EM; Wilson-WB
Source
Arch Environ Contam Toxicol 1999 Oct; 37(3):283-289
NIOSHTIC No.
20032353
Abstract
A low-variability method to reactivate blood cholinesterases (ChEs) after prior exposure of mammals, including humans, to ChE-inhibiting organophosphate esters (OPs) is presented. A concentration of 10 mM pyridine 2-aldoxime methochloride (2-PAM Cl) was incubated with intact red blood cells (RBCs) and assayed virtually free of interfering oxime and hemoglobin (Hb). Variability was decreased by reducing the number of washing steps and sedimenting RBC ghosts through a 7% sucrose cushion. Statistically significant detections of reactivations as low as 5% with average "false positives" of 3.8% were achieved. Relative rates and extent of reactivation after OP treatment of rabbit RBC AChE in vitro were of the order dimethyl- (DDVP) > diethyl- (ethyl paraoxon) >, diisopropyl-substituted (diisopropyl fluorophosphate; DFP) OPs. Rabbit RBC AChE was reactivatable for up to 60 h following dermal exposure to ethyl parathion and reactivatable for only 12 to 24 h following exposure to methyl parathion. Reactivation of plasma ChEs with 0.1 mM 2-PAM Cl in the same animals was achievable for only 12 to 24 h after ethyl parathion and for only 1 to 4 h after methyl parathion.
Keywords
Blood-analysis; Blood-disorders; Blood-samples; Exposure-assessment; Exposure-levels; Chemical-analysis; Biological-monitoring; Biomarkers
Contact
B. W. Wilson, University of California at Davis, Department of Animal Sciences and Department of Environmental Toxicology, 4209 Meyer Hall, Davis, California 95616, USA
CODEN
AECTCV
CAS No.
55-91-4
Publication Date
19991001
Document Type
Journal Article
Funding Amount
279815
Funding Type
Grant
Fiscal Year
2000
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-CCR-115818
Issue of Publication
3
ISSN
0090-4341
Priority Area
Work Environment and Workforce: Mixed Exposures
Source Name
Archives of Environmental Contamination and Toxicology
State
CA; MA
Performing Organization
Harvard Medical School, Boston, Massachusetts
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