Application of an enzyme-linked immunosorbent assay for glial fibrillary acidic protein as an indicator of the presence of brain or spinal cord in meat.
Schmidt-GR; Yemm-RS; Childs-KD; O'Callaghan-JP; Hossner-KL
Abstr Pap - Am Chem Soc 2001 Aug; 222(Part 1):U59
The presence of brain or spinal cord as an inadvertent contaminant of meat may result from the stunning of livestock or the preparation of advanced meat, recovery meat from vertebral column. If the bovine spongiform encephalopathy (BSE) agent was present in the animals being processed, this could be a food safety concern. The current methods to detect central nervous system (CNS) tissue in blood, lungs or meat are cumbersome, time consuming and costly. The objective of this study was to use glial fibrillary acidic protein (GFAP), which is restricted to the CNS, in an enzyme-linked immunosorbent assay (ELISA) for the detection of CNS tissue in blood and meat products from beef cattle. We report the development and validation of a fluorescent ELISA for GFAP which can be used as a rapid and sensitive method to detect the presence of CNS tissue in meat products. The fluorometric assay was sensitive to 0.2 ng GFAP, has an intra-assay coefficient of variation (CV) of 2.0o/Q and an interassay CV of 14.1%. Less than 1.0 ng GFAP/mg tissue was found on most beef subprimals and advanced meat recovery (AMR) product. The presence of sausage ingredients or heating the product to 80°c for 60 minutes did not affect GFAP detection. However, heating the product to 115°C for 100 minutes eliminated the detectability of the GFAP.
Food-contaminants; Food; Contaminated-food; Central-nervous-system; Central-nervous-system-disorders; Enzyme-activity; Enzymes; Brain-matter; Spinal-cord
G. R. Schmidt, Department of Animal Aciences, Colorado State University, Fort Collins, CO 80523-1171
Abstract; Conference/Symposia Proceedings
Abstracts of Papers - American Chemical Society