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Molecular and morphologic determinanats of organic dust exposures in alveolar macrophage (AM) cells.

Authors
Cosma-GN; Davis-WF; Ufferfilge-JE; Olenchok-SA; Beard-ML
Source
Toxicologist 1995 Mar; 15(1):96
NIOSHTIC No.
20032268
Abstract
To understand the pathology of pulmonary diseases after exposures to airborne dusts, we have previously measured acute-phase gene induction in rat/mouse lung after inhalation of respirable particulates. We have now measured in vitro responses of target AM cells to grain dust (GD) extracts and specific etiologic agents (bacterial endotoxin and fungal mycotoxins) found in elevator wheat samples. Area samples of respirable wheat dust were collected onto filters by personal pumps during routine elevator operations. Endotoxin levels were, quantitated by chromogenic LAL assay, and fungi identified by standard mycologic culture. Rat AM cell line NR8383 displayed greatly reduced cell growth and elevated toxicity in response to purified endotoxin vs. Alternaria mycotoxins. When cells were exposed to G D extracts, dose-responses most closely resembled those of endotoxin alone, suggesting a pivotal role for this febrile agent in the pathophysiology of dust-related pulmonary diseases, induction of the metallothionein gene was also detected by Northern blot analyses. Further acute-phase gene induction and oxidative burst analyses are in progress.
Keywords
Laboratory-animals; Animal-studies; Dust-particles; Dust-exposure; Particulate-dust; Airborne-particles; Pulmonary-system-disorders; Respiratory-system-disorders; Respirable-dust; Lung-irritants; Lung-disorders; Organic-dusts; Bacterial-dusts; Fungi
Publication Date
19950301
Document Type
Abstract
Funding Amount
974184
Funding Type
Cooperative Agreement
Fiscal Year
1995
NTIS Accession No.
NTIS Price
Identifying No.
Cooperative-Agreement-Number-U07-CCU-807121
Issue of Publication
1
ISSN
0731-9193
Source Name
The Toxicologist. Society of Toxicology 34th Annual Meeting, March 5-9,1995, Baltimore, Maryland
State
CO; WV
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