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Grid-immunoplotting: a fast and simple technique to test multiple allergens with small amounts of antibody for cross-reactivity.

Authors
Reese-G; Schmechel-D; Ayuso-R; Lehrer-SB
Source
J Chromatogr B 2001 May; 756(1-2):151-156
NIOSHTIC No.
20032156
Abstract
Grid-immunoblotting is a procedure that allows the simultaneous testing of up to 20 different antibodies such as monoclonal antibody-containing hybridoma supernatants or human sera for specific antibodies to up to 20 different antigens or allergens on a single sheet of nitrocellulose membrane. Since only 150 to 200 mul of antibody-containing solution are required this technique is uniquely suited to test growing hybridomas and small amounts of sera (e.g. mouse and children's sera). Compared to a standard ELISA, approximately ten times less antibody is needed to obtain the same information.
Keywords
Immunological-tests; Immunology; Allergies; Allergic-reactions; Biological-agents; Blood-analysis; Blood-sampling; Blood-serum; Blood-tests
CODEN
JCBADL
Publication Date
20010525
Document Type
Journal Article
Email Address
greese@tulane.edu
Fiscal Year
2001
NTIS Accession No.
NTIS Price
Issue of Publication
1-2
ISSN
0378-4347
NIOSH Division
HELD
Source Name
Journal of Chromatography B: Biomedical Applications
State
LA; WV
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