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Urinary 2-butoxyacetic acid: development of an effective gas chromatographic test method for quantification.

Authors
B'Hymer-C; Cheever-KL
Source
Toxicologist 2007 Mar; 96(1):85
NIOSHTIC No.
20031903
Abstract
A simple and effective general test method for 2-butoxyacetic acid (2-BAA) in urine samples was developed. 2-Butoxyacetic acid is a metabolite and biomarker for exposure to 2-butoxyethanol (2-BE), a glycol ether and is of concern because of the general toxicity of this class of compounds. Glycol ethers have been frequently reported to damage the male reproductive system, hematopietic system, and fetal/embryonic development. Occupational exposure by these widely used glycol ethers is likely, since they are readily absorbed through the skin. Specifically, 2-BE is used as a component in paints, inks and common house hold cleaning products, and is of interest to this laboratory for exposed populations. For the use of this test on urine, specimens were first spiked with deuterated 2-BAA; the deuterated analog was used as a procedural internal standard. The samples were extracted with ethyl acetate, concentrated, and treated by acid catalyzed esterification to produce the corresponding ethyl esters of 2-BAA. Subsequently, the ethyl ester derivatives were extracted using methylene chloride and concentrated to produce the final solution for gas chromatographic analysis. A mass selective detector (MSD) using a 50-m X 0.20-mm (id) HP-1 capillary column and a temperature program of 60 to 150oC was used for the gas chromatographic measurement. Ion m/z 57 was monitored for the ethyl ester of 2-BAA and ion m/z 66 was monitored for the internal standard. A series of recovery studies using 1, 5, 10 and 20 g/ml 2-BAA spiked urine samples demonstrated good accuracy and precision; recovery varied between 100-102% of theory. The limit of detection (LOD) was found to range from 0.005 to 0.015 g/ml for this analysis method.
Keywords
Cell-alteration; Cellular-reactions; Cell-biology; Cell-function; Cell-metabolism; Cell-morphology; Cell-transformation; Epidemiology; Biomarkers; Urinalysis; Urine-chemistry; Reproductive-system-disorders; Chemical-reactions; Chemical-properties; Chemical-composition
CAS No.
2516-93-0; 141-78-6
Publication Date
20070301
Document Type
Abstract
Fiscal Year
2007
NTIS Accession No.
NTIS Price
Issue of Publication
1
ISSN
1096-6080
NIOSH Division
DART
Source Name
The Toxicologist. Society of Toxicology 46th Annual Meeting and ToxExpo, March 25-29, 2007, Charlotte, North Carolina
State
OH
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