An immunoassay for a urinary metabolite as a biomarker of human exposure to the pyrethroid insecticide permethrin.
Ahn-KC; Ma-SJ; Tsai-HJ; Gee-SJ; Hammock-BD
Anal Bioanal Chem 2006 Feb; 384(3):713-722
Permethrin is the most popular synthetic pyrethroid insecticide used in agriculture and public health. For the assessment of human exposure to permethrin, a competitive indirect enzyme-linked immunosorbent assay (ELISA) for the detection of the glycine conjugate of a major metabolite, cis-/trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane-1-carboxylic acid (DCCA), of permethrin was developed based on a polyclonal antibody. An assay based on an antibody with a high sensitivity was optimized and characterized. The IC50 value and the detection range for trans-DCCA-glycine, in the assay buffer were 1.2 and 0.2-7.0 microg/L, respectively. The antibody recognized trans-DCCA-glycine and the mixture of cis-/trans-DCCA-glycine with an isomer range from 30:70 to 50:50 nearly equally. Little or no cross-reactivity to permethrin and its other free metabolites or glycine conjugates was measured. The integration of the ELISA and solid-phase extraction which was used to reduce the matrix effect from human urine samples provided for analysis of total cis-/trans-DCCA-glycine at low parts per billion levels in the samples. The limit of quantitation of the target analyte was 1.0 microg/L in urine with a limit of detection of 0.1 microg/L in buffer. This assay might be a useful tool for monitoring human exposure to permethrin.
Agriculture; Agricultural-workers; Agricultural-industry; Respiratory-system-disorders; Workers; Worker-health; Dusts; Dust-particles; Bronchial-asthma; Occupational-exposure; Urine-chemistry; Urinalysis; Humans; Pulmonary-disorders; Pulmonary-system; Pulmonary-system-disorders
Bruce D. Hammock, Department of Entomology and UCD Cancer Research Center, University of California, Davis, CA 95616
Agriculture; Cooperative Agreement
Analytical and Bioanalytical Chemistry
University of California - Davis