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Identification of a novel domain at the N terminus of caveolin-1 that controls rear polarization of the protein and caveolae formation.

Authors
Sun-XH; Flynn-DC; Castranova-V; Millecchia-LL; Beardsley-AR; Liu-J
Source
J Biol Chem 2007 Mar; 282(10):7232-7241
NIOSHTIC No.
20031872
Abstract
When cells are migrating, caveolin-1, the principal protein component of caveolae, is excluded from the leading edge and polarized at the cell rear. The dynamic feature depends on a specific sequence motif that directs intracellular trafficking of the protein. Deletion mutation analysis revealed a putative polarization domain at the N terminus of caveolin-1, between amino acids 32-60. Alanine substitution identified a minimal sequence of 10 residues ((TKEIDLVNRD55)-T-46) necessary for caveolin-1 rear polarization. Interestingly, deletion of amino acids 1-60 did not prevent the polarization of caveolin-1 in human umbilical vein endothelial cells or wild-type mouse embryonic fibroblasts because of an interaction of Cav(61-178) mutant with endogenous caveolin-1. Surprisingly, expression of the depolarization mutant in caveolin-1 null cells dramatically impeded caveolae formation. Furthermore, knockdown of caveolae formation by methyl-beta-cyclodextrin failed to prevent wild-type caveolin-1 rear polarization. Importantly, genetic depletion of caveolin-1 led to disoriented migration, which can be rescued by full-length caveolin-1 but not the depolarization mutant, indicating a role of caveolin-1 polarity in chemotaxis. Thus, we have identified a sequence motif that is essential for caveolin-1 rear polarization and caveolae formation.
Keywords
Cell-biology; Cell-function; Cell-transformation; Cellular-structures; Chemical-reactions; Chemical-synthesis; Chemoreceptors; Genes; Genetic-disorders; Genotoxic-effects; Genotoxicity; Genetic-factors; Gene-mutation
Contact
Jun Liu, West Virginia University Health Science Center, Dept. of Physiology and Pharmacology, P.O. Box 9229, Morgantown, WV 26506-9229
CODEN
JBCHA3
Publication Date
20070309
Document Type
Journal Article
Email Address
junliu@hsc.wvu.edu
Fiscal Year
2007
NTIS Accession No.
NTIS Price
Issue of Publication
10
ISSN
0021-9258
NIOSH Division
HELD
Priority Area
Manufacturing
Source Name
Journal of Biological Chemistry
State
WV
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