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Development, validation and use of a fluorescent covalent microsphere immunoassay (FCMIA) for the measurement of IgG antibodies to Bacillus anthracis protective antigen in human sera.

Authors
Biagini-RE; Sammons-DL; Smith-JP; Snawder-JE; Striley-CAF; MacKenzie-BA; Quinn-CP
Source
Abstr Pap - Am Chem Soc 2003 Sep; 226(Part 1):U93 066-AGRO
NIOSHTIC No.
20031844
Abstract
At the request of the Environmental Protection Agency, we performed immuno­logical biological monitoring for exposure to Bacillus anthracis (Ba) on workers conducting environmental sampling in response to the October, 2001 terror attack at the American Media, Inc., building In Boca Raton, FL. We developed, validated and used a fluorescent covalent microsphere immunoassay (FCMIA) to measure anti-Ba-protective antigen (PA) IgG in workers' sera. The FCMIA gave 4 positive anti-PA IgG results. The positive sera were the pre-entry and follow-up samples of two workers who had received their last dose of anthrax vaccine In 2000. We also compared the FCMIA to a Centers for Disease Control standardized enzyme-linked immunosorbent assay (ELISA) for anti-PA IgG (Emerg Infect Dis 8:1103-10, 2002). A high positive correlation (R=0.923, P <0.001) was observed when 20 other samples were co-analyzed for anti-PA IgG in a double-masked analysis using FCMIA and ELISA.
Keywords
Exposure-levels; Exposure-assessment; Blood-analysis; Antibody-response; Serological-techniques; Bacteria; Bacterial-disease; Environmental-contamination; Environmental-exposure; Environmental-hazards
Contact
Biomonitorlng and Health Assessment Branch, CDC/NIOSH, 4676 Columbia Parkway, Mailstop C-26, Cincinnati, OH 45226,
CODEN
ACSRAL
Publication Date
20030901
Document Type
Abstract; Conference/Symposia Proceedings
Fiscal Year
2003
NTIS Accession No.
NTIS Price
ISSN
0065-7727
NIOSH Division
DART
Source Name
Abstracts of Papers - American Chemical Society
State
OH; GA
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