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Transforming growth factor-beta following skeletal muscle strain injury in rats.

Authors
Smith-CA; Stauber-F; Waters-C; Alway-SE; Stauber-WT
Source
J Appl Physiol 2007 Feb; 102(2):755-761
NIOSHTIC No.
20031762
Abstract
Transforming growth factor-beta (TGF-beta) is a multifunctional cytokine implicated in inflammatory processes, wound healing, and fibrosis. In muscle diseases (i.e., dystrophy and inflammatory myopathy) and in animal models of muscle injury (i.e., produced by cardiotoxin, laceration, and eccentric contractions), increased TGF-beta was associated with muscle fibrosis and healing. Although TGF-beta transcript abundance was increased following injury, many studies presume that TGF-beta protein was also active as evident by increases in collagen transcript abundance. The purpose was to determine whether TGF-beta protein is present and active 48 h following injury. Using female rats, muscle strains were produced by stretching (50 stretches) the plantar flexor muscles. Forty-eight hours following injury, the medial gastrocnemius was removed and compartmentalized into five equal segments. Damaged myofibers with intracellular concanavalin A staining were counted. The percentage of damaged myofibers was significantly greater in the distal-most segment. TGF-beta was assessed by using immunohistochemistry, RT-PCR, and immunoblot analysis. Immunohistochemistry revealed the presence of TGF-beta1 in areas of myofiber injury, whereas TGF-beta2 was not detected. Increases in TGF-beta1 and TGF-beta2 transcript abundance following strain injury were documented by RT-PCR analysis. Increases in TGF-beta1 and TGF-beta2 precursor abundance were observed following strain injury by using immunoblot analysis but there was no change in active TGF-beta abundance. Although there was no correlation between the amount of cellular injury and TGF-beta transcript and protein abundance, elevated levels of TGF-beta1 and TGF-beta2 precursor proteins were present in strain-injured skeletal muscles 48 h after injury.
Keywords
Muscles; Musculoskeletal-system; Skeletal-system; Injuries; Muscle-tissue; Musculoskeletal-system-disorders; Animal-studies; Myeloid-tissue; Fibrogenicity; Fibrosis; Muscle-cells; Muscle-physiology; Muscle-stress
CODEN
JAPHEV
Publication Date
20070201
Document Type
Journal Article
Funding Amount
868663
Funding Type
Grant
Fiscal Year
2007
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-002918
Issue of Publication
2
ISSN
8750-7587
Priority Area
Disease and Injury: Musculoskeletal Disorders of the Upper Extremities
Source Name
Journal of Applied Physiology
State
WV
Performing Organization
West Virginia University, Morgantown, West Virginia
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