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Glucose partition coefficient and diffusivity in the lower skin layers.

Authors
Khalil-E; Kretsos-K; Kasting-GB
Source
Pharm Res 2006 Jun; 23(6):1227-1234
NIOSHTIC No.
20031615
Abstract
PURPOSE: This work aims to estimate the diffusivity and partitioning of glucose in the dermis and the viable epidermis of human skin. METHODS: The partition coefficient of glucose between phosphate-buffered saline and dermis, tape-stripped epidermis (TSE), stratum corneum (SC), and split-thickness skin, was measured in vitro using human cadaver skin. Glucose permeability across dermis and tape-stripped split-thickness skin (TSS) was measured using side-by-side diffusion cells. Glucose desorption from TSE and human epidermal membrane (HEM) was measured. All measurements were conducted at 32 degrees C. RESULTS: The partition coefficient for glucose [mean +/- SD (no. of samples)] was 0.65 +/- 0.09 (n = 25) for dermis, 0.81 +/- 0.06 (n = 10) for TSE, and 0.53 +/- 0.12 (n = 9) for SC. Glucose diffusivity in dermis was calculated to be 2.64 +/- 0.42 x 10(-6) cm2/s (n = 14). Glucose diffusivities in the viable epidermis estimated from TSS permeation, TSE desorption, and HEM desorption were 0.075 +/- 0.050 x 10(-6) cm2/s (n = 5), 0.037 +/- 0.018 x 10(-6) cm2/s (n = 4), and 1.0 +/- 0.6 x 10(-6) cm2/s (n = 4), respectively. CONCLUSION: The tissue/buffer partition coefficient of glucose in all skin layers was found to be less than unity, suggestive of excluded volumes in each layer. Glucose diffusivity in human dermis was found to be one third of its value in water, indicative of hindered diffusion related to the structural components of the tissue. A substantially lower value for glucose diffusivity in viable epidermis is suggested.
Keywords
Diffusion-analysis; Laboratory-animals; Animals; Animal-studies; Humans; In-vitro-study; In-vitro-studies; Skin; Cytology; Dermatology; Skin-tests
Contact
Faculty of Pharmacy, The University of Jordan, Amman, 11942, Jordan
CODEN
PHREEB
Publication Date
20060601
Document Type
Journal Article
Email Address
ekayoub@ju.edu.jo
Funding Type
Grant
Fiscal Year
2006
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-007529
Issue of Publication
6
ISSN
0724-8741
Priority Area
Research Tools and Approaches: Exposure Assessment Methods
Source Name
Pharmaceutical Research
State
OH
Performing Organization
University of Cincinnati
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