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Use of liquid suspension array technology to measure environmental, bioterrorism, and immunodiagnostic analytes.

Authors
Biagini-RE; Sammons-DL; Smith-JP; Striley-CAF; MacKenzie-BA; Snawder-JE; Robertson-SA
Source
Picogram 2006 Fall; (71):120
NIOSHTIC No.
20031602
Abstract
Luminex® multianalyte liquid suspension array profiling (Xmap®) technology can be used to develop fluorescent covalent microsphere immunoassays (FCMIAs) for numerous types of analytes using indirect, competitive, capture/sandwich as well as other assay formats. We have described FCMIAs for serologic markers to 5 CDC select agents (Bacillus anthracis,Yersinia pestis, Francisella tularensis, ricin toxin, and staphylococcal enterotoxin B; Analytical and Bioanalytical Chemistry 382:1027-34, 2005), IgG antibodies to anthrax toxins (Clin Diag Lab Immunol 11:50-55, 2004), IgG antibodies to 23 pneumococcal polysacchrides (Clin Diag Lab Immunol, 10:744-750, 2003), 3 pesticides/metabolites (Analytical and Bioanalytical Chemistry, 379:368-374, 2004), genetically-modifiedorganism pesticidal proteins (Cry1Ab and Cry3B) and 25 cytokines (IL-1â, IL-1ra, IL-2, IL-2R, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12p40, IL-13, IL-15, IL-17, TNF-á, IFN- á, IFN-ã, GM-CSF, MIP-1- á, MIP-1-â, IP-10, MIG, Eotaxin, RANTES, and MCP-1). In general, FCMIAs are faster, more sensitive, use less sample, are more precise, and have higher throughput than any competing assay technology. In this presentation, we describe the performance of these analyses and their usefulness as diagnostic and environmental methods, comparing them to traditional enzyme-linked, immunosorbant assays (ELISAs) and classical instrumental methods, such as gas- and high performance liquid chromatography.
Keywords
Immunological-tests; Immunology; Analytical-processes; Bioassays
Contact
BHAB, Centers for Disease Control and Prevention/NIOSH, 4676 Columbia Parkway, Cincinnati, OH 45226
Publication Date
20061021
Document Type
Abstract; Conference/Symposia Proceedings
Email Address
reb4@cdc.gov
Fiscal Year
2007
NTIS Accession No.
NTIS Price
Issue of Publication
71
NIOSH Division
DART
Source Name
Picogram. American Chemical Society 232st National Meeting & Exposition September 10 - 14, San Francisco, California
State
OH; CA
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