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Immunomodulatory effect of endotoxin on the development of latex allergy.

Authors
Howell-MD; Tomazic-VJ; Leakakos-T; Truscott-W; Meade-BJ
Source
J Allergy Clin Immunol 2004 May; 113(5):916-924
NIOSHTIC No.
20031583
Abstract
Background: Although numerous studies have been conducted delineating the clinical manifestations of latex allergy and characterizing the protein allergens, little is known regarding the natural history of the disease. Objective: These studies were undertaken to investigate the immunomodulatory role of inhaled endotoxin on the development of latex-specific IgE-mediated responses to natural rubber latex (NRL) proteins by using a mouse model. Methods: Female BALB/c mice were exposed to 25 mug of NRL proteins with or without increasing concentrations of endotoxin (50-25,000 EU) through the respiratory tract. Serum antibody levels were evaluated biweekly during the study. After sensitization, mice were challenged with methacholine or NRL proteins, and airway hyperreactivity (AHR) was evaluated with whole-body plethysmography. After NRL challenge, lungs were excised for histopathology, and lung-associated lymph nodes were removed for cytokine mRNA evaluation. Results: When compared with mice exposed to latex alone, mice exposed to latex and endotoxin demonstrated up to 50% lower levels of latex-specific IgE and decreased latex-specific AHR and mucin production. Conversely, these same animals demonstrated increased levels of latex-specific serum IgG2a and IgA antibodies and an increase in IFN-gamma and IL-12 mRNA levels in the draining lymph node cells. Concurrent exposure to LPS with nonammoniated latex resulted in increased alveolitis and nonspecific AHR on respiratory challenge with methacholine. Conclusion: Coexposure with LPS and allergen decreased latex-specific IgE but augmented nonspecific AHR. These studies demonstrate that endotoxin associated with NRL gloves can modulate the development of allergic responses to NRL proteins.
Keywords
Autoimmunity; Inhalation-studies; Pulmonary-disorders; Lung-disease; Lung-disorders; Lung-irritants; Animal-studies; Allergens; Allergic-reactions; Allergies
Contact
BJ Meade, MS 4020,1095 Willowdale Rd, Morgantown, WV 26505
CODEN
JACIBY
Publication Date
20040423
Document Type
Journal Article
Fiscal Year
2004
NTIS Accession No.
NTIS Price
Issue of Publication
5
ISSN
0091-6749
NIOSH Division
OD
Source Name
Journal of Allergy and Clinical Immunology
State
WV; CA; GA
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