Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Recapitulation of cell signaling events associated with astrogliosis using the brain slice preparation.

Authors
Damiani-CL; O'Callaghan-JP
Source
J Neurochem 2007 Feb; 100 (3):720-726
NIOSHTIC No.
20031553
Abstract
Astroglial activation constitutes a dominant response to all types of injuries of the CNS. Despite the ubiquitous nature of this cellular reaction to neural injury, a little is known concerning the signaling mechanisms that initiate it. Recently, we demonstrated that astrocytic hypertrophy and enhanced expression of glial fibrillary acidic protein resulting from toxicant-induced neurodegeneration are linked to activation of the janus kinase (JAK)-signal transducer and activator of transcription-3 (STAT3) pathway. These observations implicate ligands at the gp130 receptor as potential upstream effectors of astrogliosis. Here we used the brain slice preparation to examine potential activators of the JAK-STAT3 pathway. Following incubation of freshly cut striatal slices in phosphate-free oxygenated buffer for up to 75 min, we found that slicing the striatum itself was a sufficient stimulus to initiate a rapid activation of the JAK-STAT3 pathway as assessed with immunoblots of pSTAT3((tyr705)) using phospho-state specific antibodies. The mRNA for the gp130 cytokines, leukemia inhibitory factor, interleukin-6 and oncostatin M or the beta-chemokine, monocyte chemoattractive protein (CCl2) also were up-regulated in the slice. Moreover, we could enhance the activation of STAT3((tyr705)) by adding exogenous cytokines to the slice and we could inhibit phosphorylation of STAT3((tyr705)) by addition of tyrosine kinase inhibitors (Lav A and AG490) or neutralizing antibodies directed against leukemia inhibitory factor or oncostatin M. These data suggest that STAT3 activation is an early event in slice-induced glial activation and establishes the brain slice preparation method as a reliable model to examine the signaling mechanisms that underlie glial activation.
Keywords
Nervous-system-function; Nervous-system-disorders; Neurological-reactions; Neurological-system; Brain-disorders; Brain-matter; Leukemogenesis; Leukocytes; Cancer
Contact
JP O'Callaghan JP, NIOSH, Ctr Dis Control & Prevent, CDC, M-S L-3014,1095 Willowdale Rd, Morgantown, WV 26505
CODEN
JONRA9
Publication Date
20070201
Document Type
Journal Article
Email Address
jdo5@cdc.gov
Fiscal Year
2007
NTIS Accession No.
NTIS Price
Issue of Publication
3
ISSN
0022-3042
NIOSH Division
HELD
Source Name
Journal of Neurochemistry
State
WV
TOP