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Nanoindentation differentiates tissue-scale functional properties of native articular cartilage.

Authors
Li-C; Pruitt-LA; King-KB
Source
J Biomed Mater Res A 2006 Sep; 78(4):729-738
NIOSHTIC No.
20031154
Abstract
Cartilage mechanical properties are typically tested at the macroscale. To demonstrate the ability of nanoindentation to characterize in situ articular cartilage properties at the tissue scale, we investigated the local structure-property relationships of intact articular cartilage of a normal rabbit metacarpophalangeal joint. We calculated the mechanical parameters of stiffness, S, resistance to penetration, R, and volumetric creep strain, dV/V, from nanoindentation of the articular surface at specific regions of interest. We measured morphological parameters of superficial zone thickness, middle zone thickness, total uncalcified thickness, and cell density from corresponding regions with light and polarized light microscopy. Mechanical parameters were compared to morphological parameters. There were significant positive correlations (r = 0.98, p < 0.05) between superficial zone thickness and both S and R. However, we found no significant correlation between dV/V and the zone sizes. There were moderate, negative correlations between cell density and both S and R, suggesting an effect of cell volume on cartilage behavior at the tissue scale. We opine that the superficial zone plays important role in load support, as evidenced by correlations between zone size and intact cartilage mechanical properties.
Keywords
Biomechanics; Animals; Animal-studies; Cell-differentiation; Cytology; Laboratory-animals; Cell-cultures
Publication Date
20060915
Document Type
Journal Article
Email Address
Karen.King@ucsf.edu
Funding Type
Grant
Fiscal Year
2006
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-T42-CCT-910427; Grant-Number-R01-OH-007786
Issue of Publication
4
ISSN
1549-3296
Priority Area
Disease and Injury: Musculoskeletal Disorders of the Upper Extremities
Source Name
Journal of Biomedical Materials Research. Part A
State
CA; CO
Performing Organization
Northern California Educaton and Research Center, Center for Occupational and Environmental Health, University of California, School of Public Health, Berkeley, California
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