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Detection of allergens from Alternaria alternata by gold-conjugated anti-human IgE and field emission scanning electron microscopy.

Authors
Sercombe-JK; Eduard-W; Romeo-TC; Green-BJ; Tovey-ER
Source
J Immunol Methods 2006 Oct; 316(1-2):167-170
NIOSHTIC No.
20031143
Abstract
Fungal allergens are present in viable and non-viable conidia, hyphae and fungal fragments. It has been shown that large quantities of allergen are released from conidia during germination. We used a gold immunolabelling technique and field emission scanning electron microscopy to examine the allergen release from Alternaria alternata conidia. Immunolabelling was associated with the hyphal tip and amorphous matter associated with the emerging hyphae. Non-specific antibody controls showed no labelling associated with germinating fungi. This suggests that material released from hyphae may be an additional source of fungal allergens.
Keywords
Immunology; Allergens; Microscopy; Fungi; Fungicides; Fungal-infections; Fungal-diseases; Microorganisms
Contact
PO Box 8149, NO-0033 Oslo, Norway
CODEN
JIMMBG
Publication Date
20061030
Document Type
Journal Article
Email Address
wijnand.eduard@stami.no
Fiscal Year
2007
NTIS Accession No.
NTIS Price
Issue of Publication
1-2
ISSN
0022-1759
NIOSH Division
HELD
Priority Area
Research Tools and Approaches: Exposure Assessment Methods
Source Name
Journal of Immunological Methods
State
WV
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