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A two-stage cyclone using microcentrifuge tubes for personal bioaerosol sampling.

Authors
Lindsley-WG; Schmechel-D; Chen-BT
Source
J Environ Monit 2006 Nov; 8(11):1136-1142
NIOSHTIC No.
20031125
Abstract
Personal aerosol samplers are widely used to monitor human exposure to airborne materials. For bioaerosols, interest is growing in analyzing samples using molecular and immunological techniques. This paper presents a personal sampler that uses a two-stage cyclone to collect bioaerosols into disposable 1.5 ml Eppendorf-type microcentrifuge tubes. Samples can be processed in the tubes for polymerase chain reaction (PCR) or immunoassays, and the use of multiple stages fractionates aerosol particles by aerodynamic diameter. The sampler was tested using fluorescent microspheres and aerosolized fungal spores. The sampler had first and second stage cut-off diameters of 2.6 Ám and 1.6 Ám at 2 l min-1(geometric standard deviation, GSD = 1.45 and 1.75), and 1.8 Ám and 1 Ám at 3.5 l min-1(GSD = 1.42 and 1.55). The sampler aspiration efficiency was 98% at both flow rates for particles with aerodynamic diameters of 3.1 Ám or less. For 6.2 Ám particles, the aspiration efficiency was 89% at 2 l min-1 and 96% at 3.5 l min-1. At 3.5 l min-1, the sampler collected 92% of aerosolized Aspergillus versicolor and Penicillium chrysogenum spores inside the two microcentrifuge tubes, with less than 0.4% of the spores collecting on the back-up filter. The design and techniques given here are suitable for personal bioaerosol sampling, and could also be adapted to design larger aerosol samplers for longer-term atmospheric and indoor air quality sampling.
Keywords
Aerosols; Fungi; Sampling; Sampling-equipment; Sampling-methods
Contact
Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505
CODEN
JEMOFW
Publication Date
20061101
Document Type
Journal Article
Email Address
WLindsley@cdc.gov
Fiscal Year
2007
NTIS Accession No.
NTIS Price
Issue of Publication
11
ISSN
1464-0325
NIOSH Division
HELD
Priority Area
Disease and Injury: Infectious Diseases
Source Name
Journal of Environmental Monitoring
State
WV
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