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Rapid detection and quantitation of fungal spores from dust samples using real-time PCR.

Authors
Keswani-J; Kashon-M; Chen-B
Source
Bioaerosols, Fungi, Bacteria, Mycotoxins and Human Health: Patho-physiology, Clinical Effects, Exposure Assessment, Prevention and Control in Indoor Environments and Work. Johanning E, ed., Albany, New York: Fungal Research Group Foundation, Inc., 2005 Mar; 366-374
Link
NIOSHTIC No.
20030922
Abstract
Recent advances in real-time PCR have permitted accurate, rapid and quantitative identification of microorganisms in pure cultures regardless of viability or culturability. In this study, a simple sample processing method was investigated for rapid identification and quantitation of fungal spores from dust samples using real-time PCR. The proposed method was evaluated for susceptibility to interference from environmental dust samples. The extent of inhibition was calculated using real-time PCR reactions containing Aspergillus fumigatus spores specific primers and probe and various amounts of dust. No interference (p < 0.05) was detected from 0.2 mg of four real-world dust samples. However, dusts weighing > 0.2 mg compromised the assay. The overall results suggest the potential usefulness of our method for monitoring indoor microbial aerosols containing dusts weighing < / = 0.2 mg using real-time PCR.
Keywords
Fungi; Fungicides; Dusts; Dust-particles; Dust-sampling; Microorganisms; Aerosols; Aerosol-particles; Aerosol-sampling
Publication Date
20050301
Document Type
Book or book chapter; Conference/Symposia Proceedings
Email Address
jkeswani@cdc.gov
Editors
Johanning-E
Fiscal Year
2005
NTIS Accession No.
NTIS Price
ISBN No.
0970991517
NIOSH Division
HELD
Source Name
Bioaerosols, Fungi, Bacteria, Mycotoxins and Human Health: Patho-physiology, Clinical Effects, Exposure Assessment, Prevention and Control in Indoor Environments and Work
State
WV
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