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Increased gene copy number of the transcription factor E2F1 in malignant melanoma.

Authors
Nelson-MA; Reynolds-SH; Rao-UN; Goulet-AC; Feng-Y; Beas-A; Honchak-B; Averill-J; Lowry-DT; Senft-JR; Jefferson-AM; Johnson-RC; Sargent-LM
Source
Cancer Biol Ther 2006 Apr; 5(4):407-412
NIOSHTIC No.
20030618
Abstract
Translocations and unique chromosome break points in melanoma will aid in the identification of the genes that are important in the neoplastic process. We have previously shown a unique translocation in malignant melanoma cells der(12)t(12;20). The transcription factor E2F1 maps to 20q11. Increased expression of E2F has been associated with the autonomous growth of melanoma cells, however, the molecular basis has not yet been elucidated. To this end, we investigated E2F1 gene copy number and structure in human melanoma cell lines and metastatic melanoma cases. Fluorescent in situ hybridization (FISH) analysis using a specific E2F1 probe indicated increased E2F1 gene copies in melanoma cell lines compared to normal melanocytes. We also observed increased copies of the E2F1 gene in lymph node metastases of melanoma. In addition, Western blot analysis demonstrated increased E2F1 protein levels in 8 out of 9 melanoma cell lines relative to normal melanocytes. Inhibition of E2F1 expression with RNAi also reduced melanoma cell growth. Our results suggest that the release of E2F activity by elevated E2F1 gene copy numbers may play a functional role in melanoma growth.
Keywords
Cancer; Genes; Genetics; Malignancy; Malignant-neoplasms; Cell-biology; Cell-cultures; Cell-growth
CODEN
CBTAAO
Publication Date
20060401
Document Type
Journal Article
Email Address
mnelson@azcc.arizona.edu
Fiscal Year
2006
NTIS Accession No.
NTIS Price
Issue of Publication
4
ISSN
1538-4047
NIOSH Division
HELD
Priority Area
Research Tools and Approaches: Cancer Research Methods
Source Name
Cancer Biology and Therapy
State
WV; AZ
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