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Development of a tape-stripping method to quantify dermal exposure to hexamethylene diisocyanate.

Fent-K; Jayaraj-K; Gold-A; Ball-L; Nylander-French-L
American Industrial Hygiene Conference and Exposition, May 21-26, 2005, Anaheim, California. Fairfax, VA: American Industrial Hygiene Association, 2005 May; :119
Significant worker skin contact with hexamethylene diisocyanate (HDI) occurs during the application and manufacture of surface-coatings, foams, resins, and plastics. Respiratory sensitization and occupational asthma have long been associated with airborne isocyanate exposure, but a similar association with dermal exposure to isocyanates has largely been left unexplored, mainly due to nonexistent quantitative sampling methods. We have developed a noninvasive tape-stripping technique for sampling layers of the epidermis for determination of chemical concentrations in the skin. Quantification of HDI on tape-strip samples following derivatization reaction with 1-(2-methoxyphenyl)-piperazine was performed using liquid chromatography and mass spectrometry with electrospray ionization in positive ion mode. The derivative was synthesized independently and shown to be pure by proton NMR and melting point analysis. The ion at m/z 553.4, corresponding to the protonated molecular ion, was used for quantitation. This method was tested by applying HDI-containing products to tape-strip samples, performing derivatization and quantitative analysis, and determining the yield, which ranged from 87 to 93%. The limit of detection for this method is 0.05 ?mol/uL, while the limit of quantification is 0.5 ?mol/uL. This method is highly sensitive and specific and suitable for quantification of dermal exposure to HDI in occupational settings. This method will be used to measure dermal exposure to HDI in conjunction with inhalation and biological monitoring during spray-painting operations in order to investigate the contribution of dermal exposure to total body dose.
Occupational-exposure; Skin-exposure; Resins; Plastics; Sampling; Sampling-methods; Mass-spectrometry; Ionization; Biological-monitoring; Inhalation-studies; Quantitative-analysis; Spray-painting
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American Industrial Hygiene Conference and Exposition, May 21-26, 2005, Anaheim, California
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University of North Carolina, Chapel Hill