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The use of fluorescence covalent microbead immunosorbent assays (FCMIAS) for multiplexed pesticide biomonitoring.

Authors
Smith-J; Sammons-D; Biaqini-R; Robertson-S; Striley-C; MacKenzie-B; Snawder-J
Source
2005 Toxicology and Risk Assessment Conference, April 25-28, 2005, Holiday Inn Conference Center, Fairborn, Ohio, 2005 Apr; :23
Link
NIOSHTIC No.
20030189
Abstract
Body burdens from exposures to pesticides can be estimated from urinary analyses of pesticide parent/metabolites. Pesticide applicators and others are often exposed to numerous unrelated pesticides, either sequentially or simultaneously. Classically, these analyses are performed using chemical/instrumental analysis (CIS) or enzyme linked immunosorbent assays (ELlSAs). Both of these technologies can usually measure one analyte (or closely related groups of analytes) per assay. A technology to evaluate the body burdens from these complex combined exposures simultaneously is fluorescence covalent microbead immunoassay (FCMIA), which can be multiplexed (measure numerous related or unrelated analytes simultaneously). Using FCMIA, we developed multiplexed assays for glyphosate (Gly), atrazine (Atz), metolachlor mercapturate (MM) and 2,4dichlorophenoxyacetic acid (2,4-0) in water and urine. FCMIAs are faster, use less sample, have throughput advantages, are less prone to systematic error, and have sensitivity and dynamic assay ranges similar to CIS or ELiSAs, for Gly, Atz, MM, and 2,4-0.
Keywords
Toxicology; Risk-analysis; Biological-warfare-agents; Biological-weapons; Decontamination
Publication Date
20050425
Document Type
Abstract; Conference/Symposia Proceedings
Fiscal Year
2005
NTIS Accession No.
NTIS Price
NIOSH Division
DART
Source Name
2005 Toxicology and Risk Assessment Conference, Fairborn, Ohio
State
OH
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