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Simultaneous measurement of specific serum IgG responses to five select agents.

Biagini-RE; Sammons-DL; Smith-JP; MacKenzie-BA; Striley-CAF; Robertson-SA; Snawder-JE; Quinn-CP
Fort Detrick Symposium on Diodefense and Infectious Diseases, 2005 Apr; :1
Select Agents are defined by CDC and the USDA Animal and Plant Health Inspection Service (APHIS) as biological agents or toxins deemed a threat to public, animal, or plant health, or to animal or plant products. They are classified based on their ease of dissemination, mortality/morbidity rate, and potential for social disruption. A subset of these agents includes Bacillus anthracis, Yersinia pestis, Francisella tularensis, ricin toxin (RT), and staphylococcal enterotoxin B (SEB). Infection or intoxication with these agents has been shown to elicit an antigen specific serum IgG response. We describe a fluorescent covalent microsphere immunoassay (FCMIA) for measurement of specific IgG antibodies to seven different antigens from five different select agents: B. anthracis [protective antigen (PA) and lethal factor (LF)], Y. pestis (F1 and V antigens), F. tularensis, RT and SEB simultaneously in human B. anthracis vaccinee sera (containing anti-PA and anti-LF IgG) which had been spiked with animal specific IgG antibodies to the other select agents. Inter- and intra-assay coefficients of variation were 6.5% and 13.4%, respectively (N = 4). There were no significant differences (P greater than 0.70) in assay responses when the assays were performed individually versus multiplexed. When the observed versus expected interpolated concentrations were compared using standards recovery, highly linear relationships were observed (r2 values from 0.981 to 0.999, P less than 0.001). Minimum detectable concentrations (MDCs) ranged from 0.3 ng/ml (anti-Y. pestis F1 IgG) to 300 ng/ml (anti-RT IgG). Linear dynamic ranges spanned from 125 ng/ml for anti-SEB IgG to 1333 ng/ml for anti-Y.pestis F1 IgG. The multiplexed FCMIA is considerably faster and has extremely higher throughput when compared to existing individual enzyme-linked immunosorbent assays (ELISAs) commonly used for measurement of anti-BT IgGs. These data indicate that multiplexed FCMIA is a sensitive, accurate and precise method to simultaneously measure specific IgGs in serum to CDC select agents and may be of value in screening either decontamination workers, the general population or animals for exposures/infections to these agents.
Biological-agents; Biological-monitoring; Biological-warfare-agents; Biological-weapons; Toxins; Infection-control; Infectious-diseases; Antigens; Antibody-response; Bioassays; Immunoglobulins; Decontamination; Serological-techniques
National Institute for Occupational Safety and Health, Division of Applied Research and Technology, Biomonitoring and Health Assessment Branch, 4676 Columbia Parkway, Cincinnati, OH 45226
79-41-4; 9009-86-3; 1321-74-0; 9003-53-6
Publication Date
Document Type
Interagency Agreement; Abstract; Conference/Symposia Proceedings
Email Address
Funding Type
Interagency Agreement; Construction
Fiscal Year
NTIS Accession No.
NTIS Price
NIOSH Division
Priority Area
Research Tools and Approaches: Exposure Assessment Methods
Source Name
Fort Detrick Symposium on Diodefense and Infectious Diseases, 2005 April 28-29, Fort Detrick, Maryland