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Nitrotyrosine formation in the airways and lung parenchyma of patients with asthma.

Authors
Kaminsky-DA; Mitchell-J; Carroll-N; James-A; Soultanakis-R; Janssen-Y
Source
J Allergy Clin Immunol 1999 Oct; 104(4 Pt 1):747-754
NIOSHTIC No.
20030132
Abstract
BACKGROUND: Recent evidence has shown that nitric oxide (NO) levels are increased in asthmatic airways. Although the role of NO in asthma is unknown, reactive metabolites of NO may lead to nitrotyrosine formation and promote airway dysfunction. OBJECTIVE: The aim of this study was to determine whether nitrotyrosine, as a marker of nitrating species, could be found in the airways and lung parenchyma of subjects with asthma who died of status asthmaticus or other nonrespiratory causes. METHODS: Lung tissue specimens were obtained from 5 patients who died of status asthmaticus, 2 asthmatic patients who died of nonrespiratory causes, and 6 nonasthmatic control subjects who died of nonrespiratory causes. Lung sections were stained for immunofluorescence with use of an antinitrotyrosine antibody, followed by a indiocarbocyanine (Cy5, Jackson Immunochemicals, Westgrove, Pa)-conjugated secondary antibody. RESULTS: Nonasthmatic lungs showed little or no nitrotyrosine staining, whereas asthmatic lungs demonstrated significantly more staining of nitrotyrosine residues distributed in both the airways and lung parenchyma. CONCLUSION: This study demonstrates the presence of nitrotyrosine, and hence evidence of formation of nitrating species, in the airways and lung parenchyma of patients with asthma who died of status asthmaticus or other nonrespiratory causes. This finding supports the concept that widespread airway and parenchymal inflammation occurs in asthma, and, more specifically, that NO and its reactive metabolites may play a pathophysiologic role in asthma.
Keywords
Allergens; Pathogenesis; Airway-obstruction; Animal-studies; Exposure-levels; Pulmonary-system-disorders; Respiratory-system-disorders
Contact
Division of Pulmonary Disease and Critical Care Medicine, University of Vermont College of Medicine, Burlington, VT 05405
CODEN
JACIBY
Publication Date
19991001
Document Type
Journal Article
Funding Amount
2634676
Funding Type
Grant
Fiscal Year
2000
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-003457
Issue of Publication
4
ISSN
0091-6749
NIOSH Division
HELD
Priority Area
Disease and Injury: Asthma and Chronic Obstructive Pulmonary Disease
Source Name
Journal of Allergy and Clinical Immunology
State
WV; PA; CT; CA
Performing Organization
Yale University, New Haven, Connecticut
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