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History and results of the two inter-laboratory round robin endotoxin assay studies on cotton dust.

Authors
Chun-DT; Bartlett-K; Gordon-T; Jacobs-RR; Larsson-BM; Larsson-L; Lewis-DM; Liesivuori-J; Michel-O; Milton-DK; Rylander-R; Thorne-PS; White-EM; Brown-ME; Gunn-VS; Wurtz-H
Source
Am J Ind Med 2006 Apr; 49(4):301-306
NIOSHTIC No.
20030073
Abstract
In the US cotton industry, airborne cotton dust levels are regulated, and other countries are moving to specify safety limits for airborne endotoxins. There is concern about potential respiratory health hazards associated with agricultural and other organic dusts. In laboratories, ranking which samples have high and low levels of endotoxin is usually in good agreement between laboratories. When different laboratories assay identical samples, the levels differ. The objective of this research was to evaluate the intra- and inter-laboratory variability for 13 laboratories measuring endotoxin in cotton dust. Two inter-laboratory round robin endotoxin assay studies were conducted using cotton dust. In the first round robin, each laboratory used their normal in-house assay method and then used a common extraction protocol. In the second round robin, a common extraction protocol and endotoxin assay kit was used. The intra-laboratory results had small variations but inter-laboratory results had very high variations. The inter-laboratory results using a common extraction protocol showed reduced differences. Using the same extraction protocol and endotoxin assay kit, the intra-laboratory variation was small and inter-laboratory variation was reduced but not enough for inter-laboratory agreement. Most of the laboratories were able to discern between the high and low endotoxin concentration dusts. Standardization has reduced the differences in results between laboratories and possibly further standardization may bring closer inter-laboratory agreement.
Keywords
Endotoxins; Cotton-dust; Dusts; Dust-particles; Cotton-industry; Airborne-particles; Airborne-dusts; Health-hazards; Respiratory-system-disorders; Organic-dusts; Agriculture; Agricultural-industry; Laboratory-testing; Sampling; Sampling-methods
Contact
David T.W. Chun, Microbiologist, Cotton Quality Research Station, Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 1574, Clemson, SC, 29633
CODEN
AJIMD8
Publication Date
20060401
Document Type
Journal Article
Email Address
dtwchun@mindspring.com
Fiscal Year
2006
NTIS Accession No.
NTIS Price
Issue of Publication
4
ISSN
0271-3586
NIOSH Division
DART
Priority Area
Research Tools and Approaches: Exposure Assessment Methods
Source Name
American Journal of Industrial Medicine
State
OH
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