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Application of Taqman chemistry for allelic discrimination.

Authors
Johnson-VJ
Source
Genetic Variance Detection: Technologies for Pharmacogenomics. Hecker-KH, ed., Eagleville, PA: DNA Press, 2005 Jan; :237-254
Link
NIOSHTIC No.
20029789
Abstract
Taqman chemistry has been successfully adapted for use as a tool to discriminate allelic variation in human study populations. This technique has been validated against a number of well-accepted techniques including PCR-RFLP, sequencing, and allele-specific PCR. The Taqman assays are now being used as the basis for validation of newer genotyping strategies indicating its growing acceptance in the scientific literature. These assays are amenable to the high-throughput genotyping demands of the post-human genome sequencing era and are associated with less cost and technical error compared to more traditional approaches. The application of microfluidics and nanoliter volumes will greatly further the high-throughput/low cost attributes of this genotyping platform.
Keywords
Genetics; Genotoxicity; Analytical-processes; Analytical-chemistry; DNA-damage; Bioassays; Biomarkers
Contact
Victor J. Johnson, Toxicology and Molecular Biology Branch, Health Effects Laboratory division, NIOSH/CDC, 1095 Willowdale Road, M/S 3014, Morgantown, WV 26505-2888
Publication Date
20050120
Document Type
Book or book chapter
Email Address
VJohnson3@cdc.gov
Editors
Hecker-KH
Fiscal Year
2005
NTIS Accession No.
NTIS Price
ISBN No.
0974876550
NIOSH Division
HELD
Priority Area
Disease and Injury: Asthma and Chronic Obstructive Pulmonary Disease
Source Name
Genetic Variance Detection: Technologies for Pharmacogenomics
State
WV
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