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Transforming growth factor-beta in skeletal muscle strain injury.

Authors
Smith-CA; Stauber-WT; Alway-SE; Miller-GR
Source
Med Sci Sports Exerc 2001 May; 33(5)(Suppl 1):S230
NIOSHTIC No.
20029544
Abstract
The presence of transforming growth factor-beta (TGF-beta) in skeletal muscle has been reported in various muscle disease. In this study, we assess the presence of transforming growth factor-beta in strain-induced muscle injury. Muscle strains were produced by manually stretching (50 repetitions) an activated plantar flexor group through its normal range of motion. Muscle biopsy samples were evaluated 24- and 48- hours after strain injury for the presence of transforming growth factor-beta using immunohistochemical techniques and Western immunoblot analysis. Transcript levels for collagen I, collagen III, and transforming growth factor-beta were assessed using reverse transcriptase polymerase chain reaction (RT-PCR). Immunohistochemical analysis revealed the presence of TGF-beta2 in areas of myofiber injury, whereas TGF-beta1 was in areas of cell necrosis and phagocytosis. RT-PCR indicated increases in collagen I, collagen III, and TGF-beta1 transcripts 24 hours post-strain injury. Increases in collagen I and collagen III transcripts were more pronounced 48 hours after strain injury. Western immunoblot analysis showed no apparent change in TGF-beta2 levels with injury, but an increase in TGF-beta1 levels which was most pronounced 48 hours post-strain injury. These results indicate that TGF-beta1 levels increase after acute strain injury following increases in TGF-beta1 mRNA correlating with increases in collagen I and collagen III transcripts, whereas TGF-beta2 may be a common resident in skeletal muscle awaiting activation.
Keywords
Muscles; Musculoskeletal-system; Skeletal-system; Injuries; Muscle-tissue; Injury-prevention; Musculoskeletal-system-disorders; Diseases
CODEN
MSPEDA
Publication Date
20010501
Document Type
Abstract
Funding Amount
868663
Funding Type
Grant
Fiscal Year
2001
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-002918
Issue of Publication
5
ISSN
0195-9131
Priority Area
Disease and Injury: Musculoskeletal Disorders of the Upper Extremities
Source Name
Medicine and Science in Sports and Exercise
State
WV
Performing Organization
West Virginia University, Morgantown, West Virginia
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