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Peptide sequencing by MALDI 193-nm photodissociation TOF MS.

Authors
Morgan-JW; Hettick-JM; Russell-DH
Source
Methods Enzymol 2005 Dec; 402:186-209
NIOSHTIC No.
20029492
Abstract
Ultraviolet photodissociation time-of-flight (TOF) mass spectrometry (MS) is described as a method for determination of peptide ion primary structure. Monoisotopic selection and bond-specific activation, combined with the rapidity of TOF MS analysis, render this technique invaluable to the rapidly expanding field of proteomics. Photofragment ion spectra of model peptides acquired using both post-source decay (PSD) focusing and TOF-TOF experimental methods are exhibited. Advantages of 193-nm photodissociation for de novo sequencing of peptide ions are discussed.
Keywords
Peptides; Mass-spectrometry; Enzymology; Models; Ionization; Analytical-instruments; Analytical-processes
CODEN
MENZAU
Publication Date
20051212
Document Type
Journal Article
Fiscal Year
2006
NTIS Accession No.
NTIS Price
ISBN No.
9780121828073
ISSN
0076-6879
NIOSH Division
HELD
Source Name
Methods in Enzymology
State
WV
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