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Increased levels of sperm ubiquitin correlate with semen quality in men from an andrology laboratory clinic population.

Authors
Sutovsky-P; Hauser-R; Sutovsky-M
Source
Hum Reprod 2004 Mar; 19(3):628-638
NIOSHTIC No.
20029413
Abstract
BACKGROUND: Ubiquitin, a house-keeping protein that marks other proteins for proteasomal degradation, tags defective sperm during epididymal passage. To establish ubiquitin as a biomarker of human infertility, the present study examines the relationships between sperm ubiquitin content and clinical semen parameters among men from an infertility clinic population with varied aetiologies. METHODS: Anti-ubiquitin immunoreactivity was measured by flow cytometric sperm-ubiquitin tag immunoassay (SUTI) in sperm samples of 28 infertility patients and 15 fertile donors. Semen analyses were performed by computer-assisted semen analysis and World Health Organization morphology. RESULTS: Median values of ubiquitin-induced fluorescence had a strong negative correlation with sperm count (r = -0.63, P = 0.0003) and a positive correlation with % abnormal morphology (r = 0.55, P = 0.01). Infertility patients (n = 28) had significantly higher levels of sperm ubiquitin. Out of 28 patients, six reported possible occupational exposures to solvents, three were current smokers and six were ex-smokers. Within the patient group, men with known male factor infertility, those with self-reported occupational exposure to solvents and current smokers had the highest sperm ubiquitin levels. When men with jobs involving potential occupational exposure to solvents were combined with current smokers, the highest correlations were found between sperm ubiquitin and motility (r = -0.74), count (r = -0.82) and % sperm abnormalities (r = 0.73). CONCLUSIONS: Increased sperm ubiquitin was inversely associated with sperm count, motility and % normal morphology, supporting the use of ubiquitin as a biomarker of human semen quality. SUTI assay confirmed poor semen quality in all men with poor clinical semen parameters, but also was high in some patients with seemingly good clinical semen parameters. Occupational exposure to solvents and smoking may have contributed to high levels of sperm ubiquitin in some of these patients.
Keywords
Reproductive-system-disorders; Reproductive-system; Spermatogenesis; Spermatozoa; Fertility; Mammalian-cells
Contact
Department of Animal Science, University of Missouri-Columbia, 65211-5300
CODEN
HUREEE
Publication Date
20040301
Document Type
Journal Article
Email Address
SutovskyP@missouri.edu
Funding Amount
235845
Funding Type
Grant
Fiscal Year
2004
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R21-OH-007324
Issue of Publication
3
ISSN
0268-1161
Source Name
Human Reproduction
State
OR; MO
Performing Organization
University of Missouri, Columbia, Missouri
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