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GC analysis of human breath with a series-coupled column ensemble and a multibed sorption trap.

Authors
Sanchez-JM; Sacks-RD
Source
Anal Chem 2003 May; 75(10):2231-2236
NIOSHTIC No.
20029240
Abstract
The combination of a tandem column ensemble and an on-line microsorption trap is used for the analysis of organic compounds in human breath samples. The four-bed sorption trap uses a series of discreet sorption beds containing three grades of graphitized carbon and a carbon molecular sieve to quantitatively remove most organic compounds from 0.8-L breath samples. The trap is then heated to 300 degrees C in approximately 1.5 s and maintained at this temperature for 10 s. The resulting vapor plug width is in the range 0.7-1.3 s for the compounds found in the breath samples. The separation is performed with a 15-m-long, 0.25-mm-i.d. capillary using a 0.5-microm-thick film of nonpolar dimethyl polysiloxane coupled in series to a polar column, either trifluoropropylmethyl polysiloxane or poly(ethylene glycol). Both column combinations are successful in separating the early-eluting compounds acetone, isoprene, pentane, methyl alcohol, and ethyl alcohol, which are all common in breath samples. The poly(ethylene glycol) combination gave better separation but showed relatively fast deterioration for repeated analysis of wet samples. Breath samples were obtained under different conditions (smoker, nonsmoker, gum chewer), and 25 compounds were identified in the various samples. Many additional peaks are observed but not identified. Analytical curves (log-log) of peak area versus sample volume for test compounds are linear in the range 80-800 cm3. Detection limits (3sigma) for several volatile compounds in 800-cm3 samples are in the 1-5 ppb range.
Keywords
Organic-compounds; Breathing; Sampling; Sampling-methods; Humans; Gas-chromatography; Analytical-processes; Analytical-methods; Analytical-instruments
CODEN
ANCHAM
Publication Date
20030515
Document Type
Journal Article
Funding Type
Grant
Fiscal Year
2003
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-003692
Issue of Publication
10
ISSN
0003-2700
Source Name
Analytical Chemistry
State
MI
Performing Organization
University of Michigan, Ann Arbor
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