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Hyaluronic acid binding by human sperm indicates cellular maturity, viability, and unreacted acrosomal status.

Authors
Huszar-G; Ozenci-CC; Cayli-S; Zavaczki-Z; Hansch-E; Vigue-L
Source
Fertil Steril 2003 Jun; 79(Suppl 3):1616-1624
NIOSHTIC No.
20029192
Abstract
To test, both in semen and washed-sperm fractions, whether hyaluronic acid (HA) binding is restricted to sperm that have completed cellular maturation. Comparisons of sperm in semen and in HA-bound sperm fractions. University-based diagnostic and research andrology laboratory. Semen samples originated in men being tested for infertility. The attributes of sperm maturity were tested by immunocytochemistry with creatine kinase and HspA2 antisera (highlights cytoplasmic retention in diminished-maturity sperm), aniline blue chromatin staining (detects persistent histones), pisum sativum lectin staining (reveals acrosomal integrity), and the FertiLight viability kit (highlights viable and nonviable sperm). All markers of sperm maturity and immaturity supported the hypothesis that HA-bound sperm are mature. Nonbinding sperm exhibited cytoplasmic and nuclear properties of diminished maturity. The acrosomal status of HA-bound sperm was either unreacted or slightly capacitated, but not acrosome reacted. Only viable sperm exhibited HA binding. Sperm that are able to bind to HA are mature and have completed the spermiogenetic processes of sperm plasma membrane remodeling, cytoplasmic extrusion, and nuclear histone-protamine replacement. Hyaluronic acid-bound sperm show unreacted acrosomes. These studies provide further insights into the relationship between spermiogenesis and sperm function.
Keywords
Spermatozoa; Spermatogenesis; Laboratory-testing; Acids; Humans; In-vitro-study
CODEN
FESTAS
Publication Date
20030601
Document Type
Journal Article
Email Address
gabor.huszar@yale.edu
Funding Type
Grant
Fiscal Year
2003
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-004061
ISSN
0015-0282
Source Name
Fertility and Sterility
State
CT
Performing Organization
Yale University
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