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Gene expression of primary human bronchial epithelial cells in response to coal dusts with different prevalence of coal workers' pneumoconiosis.

Authors
Hu-W; Zhang-Q; Su-WC; Feng-Z; Rom-W; Chen-LC; Tang-M; Huang-X
Source
J Toxicol Environ Health, A 2003 Aug; 66(15-16):1249-1265
NIOSHTIC No.
20029186
Abstract
Striking regional differences in the prevalence of coal workers' pneumoconiosis (CWP) have been observed but not fully understood. This study investigated the early biological responses of primary lung cells to treatment with coal dusts from various seams. High-density oligoarray technology (GeneChip, Affymetrix, Santa Clara, CA) was used to compile gene expression profiles of primary human bronchial epithelial cells to low concentrations (2 microg/cm(2)) of coals for 6 h or 24 h of treatment. Data showed that a total of 1050 out of 12,000 genes on the chip were altered by 2 coal dusts. The coal from the Pennsylvania (PA) coal-mine region with a high prevalence of CWP altered 908 genes, many more than the coal from Utah (UT) with a low prevalence of CWP, which affected 356 genes. Many genes decreased their expression levels in response to the PA coal at 6 h and/or 24 h of treatment. For example, transferrin receptor, a gene known to control cellular iron uptake, was downregulated in the cells treated with the iron-containing PA coal in order to protect cells from iron overload. The UT coal without bioavailable iron had no such effect. The downregulation patterns of genes were confirmed by reverse-transcription polymerase chain reaction (RT-PCR). This study is one of the first in profiling gene expressions of primary bronchial epithelial cells treated with coals from various seams, which may set stages for future studies on specific genes.
Keywords
Coal-dust; Dusts; Dust-particles; Coal-workers; Coal-workers-pneumoconiosis; Pneumoconiosis; Coal-mining; Coal-miners; Occupational-exposure; Inhalation-studies
CODEN
JTEHD6
Publication Date
20030808
Document Type
Journal Article
Funding Amount
638746
Funding Type
Grant
Fiscal Year
2003
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-003561
Issue of Publication
15-16
ISSN
1528-7394
Source Name
Journal of Toxicology and Environmental Health, Part A: Current Issues
State
NY
Performing Organization
New York University Medical Center, New York, New York
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