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Intracytoplasmic sperm injection: a novel selection method for sperm with normal frequency of chromosomal aneuploidies.

Authors
Jakab-A; Sakkas-D; Delpiano-E; Cayli-S; Kovanci-E; Ward-D; Ravelli-A; Huszar-G
Source
Fertil Steril 2005 Dec; 84(6):1665-1673
NIOSHTIC No.
20029128
Abstract
To test a newly invented intracytoplasmic sperm injection (ICSI) sperm selection method based on sperm hyaluronic acid (HA) binding. Comparison of chromosomal disomy and diploidy frequencies in sperm arising from semen and in HA-bound sperm. Academic andrology laboratory. Men presenting for semen analysis. Washed sperm fractions of 32 semen samples were applied to Petri dishes or glass slides coated with immobilized HA. The unbound sperm were rinsed gently, and the HA-bound sperm were removed with an ICSI pipette. The control sperm population was the unselected sperm. Both HA-selected and unselected sperm were treated with fluorescence in situ hybridization with centromeric probes for the X, Y, and 17 chromosomes. Chromosomal disomy and diploidy frequencies. In the HA-bound sperm (495-2,079 per man, 41,670 in all) compared with unselected sperm (4,770 per man, 162,210 in all), the chromosomal disomy frequencies were reduced to 0.16% from 0.52%, diploidy to 0.09% from 0.51%, and sex chromosome disomy to 0.05% from 0.27% (a 5.4-fold reduction vs. 4-fold respective increase in ICSI offspring). The HA sperm selection method for ICSI, which is based on a relationship between sperm receptors for zona pellucida and HA, will likely reduce the potential genetic complications and adverse public health effects of ICSI.
Keywords
Spermatogenesis; Spermatozoa; Demographic-characteristics; Sex-factors; Laboratory-testing; Sampling; Sampling-methods
Contact
Gabor Huszar, M.D., Yale School of Medicine, Department of Obstetrics and Gynecology, 333 Cedar Street, New Haven, Connecticut 06520
CODEN
FESTAS
Publication Date
20051201
Document Type
Journal Article
Email Address
gabor.huszar@yale.edu
Funding Type
Grant
Fiscal Year
2006
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-004061
Issue of Publication
6
ISSN
0015-0282
Source Name
Fertility and Sterility
State
CT
Performing Organization
Yale University
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