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A test procedure for the determination of (2-methoxyethoxy) acetic acid in urine from jet fuel-exposed mice.

Authors
B'Hymer-C; Keil-DE; Cheever-KL
Source
Toxicol Mech Methods 2005 Sep-Oct; 15(5):367-373
NIOSHTIC No.
20028655
Abstract
A test procedure for the determination of (2-methoxyethoxy)acetic acid (MEAA) was adapted and applied to urine samples from jet fuel (JP-8)-exposed mice using capillary gas chromatography with a mass selective detector (MSD). MEAA is a metabolite and proposed biomarker for exposure to 2-(2-methoxyethoxy)ethanol, a glycol ether component in the formulation of JP-8. The collected urine samples were spiked with deuterated butoxyacetic acid internal standard, and extracted with ethyl acetate, and esterified with ethanol and sulfuric acid, and the esters of the glycol ethers were extracted with methylene chloride. The chromatographic conditions used easily separate the MEAA ethyl ester from interferences within mouse urine. The application of this procedure to urine samples collected from mice demonstrated that MEAA was detectable after oral (2000 mg/kg) or dermal (50 u L) exposure for 7 days to JP-8 at levels as high as 8.5 or 6.5 u g/mL, respectively. This pilot demonstration indicated that total urinary MEAA was a viable biomarker for the two routes of JP-8 exposure in laboratory mice.
Keywords
Acetic-acids; Acids; Laboratory-animals; Animals; Animal-studies; Jet-engine-fuels; Urinalysis; Gas-chromatography; Exposure-levels; Exposure-assessment; Biomarkers; Sampling
Contact
U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health; Division of Applied Research and Technology, Taft Laboratory, 4676 Columbia Parkway, Cincinnati, Ohio, 45226,
CODEN
TMMOCP
CAS No.
64-19-7
Publication Date
20050901
Document Type
Journal Article
Fiscal Year
2005
NTIS Accession No.
NTIS Price
Issue of Publication
5
ISSN
1537-6524
NIOSH Division
DART; OD; HELD
Priority Area
Research Tools and Approaches: Exposure Assessment Methods
Source Name
Toxicology Mechanisms and Methods
State
OH; WV
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