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Comparison of established cell lines at different passages by karyotype and comparative genomic hybridization.

Authors
Wenger-SL; Senft-JR; Sargent-LM; Bamezai-R; Bairwa-N; Grant-SG
Source
Biosci Rep 2004 Dec; 24(6):631-639
NIOSHTIC No.
20028396
Abstract
Two established cancer cell lines, MCF-7 and Ishikawa, were both obtained directly from a cell repository and through another laboratory. The karyotypes from the two MCF-7 cell lines had up to 83 chromosomes and similarities for chromosomal gain and structural abnormalities. The two Ishikawa cell lines had up to 60 chromosomes with only a missing X as the common chromosome abnormality. CGH studies were performed by co-hybridizing the two Ishikawa or MCF-7 cell lines to normal metaphases. The differences seen between the two MCF-7 cell cultures reflect changes due to passage number and culture conditions. For Ishikawa, DNA polymorphic data and mutation studies suggest that the two cell lines are not derived from the same established tumor cell line. Our study shows the utilization of CGH in comparing cell lines originating from the same specimen. Our study also demonstrates the necessity for periodically evaluating cell lines to confirm their origin.
Keywords
Laboratory-testing; Chromosome-damage; Cell-cultures; Cell-culture-techniques; Tumors; Analytical-processes
Contact
Department of Pathology, West Virginia University, P. O. Box 9203, Morgantown, WV, 26506-9203
CODEN
BRPTDT
Publication Date
20041201
Document Type
Journal Article
Email Address
swenger@hsc.wvu.edu
Fiscal Year
2005
NTIS Accession No.
NTIS Price
Issue of Publication
6
ISSN
0144-8463
NIOSH Division
HELD
Source Name
Bioscience Reports
State
WV
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